Expressed IgH μ and τ transcripts share diversity segment in ranched Thunnus orientalis.
Expressed IgH μ and τ transcripts share diversity segment in ranched Thunnus orientalis.
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DOI：
10.1016/j.dci.2013.10.015
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发表时间：
2014-03
影响因子：
2.9
通讯作者：
Criscitiello MF
中科院分区：
文献类型：
--
作者：
Mashoof S;Pohlenz C;Chen PL;Deiss TC;Gatlin D 3rd;Buentello A;Criscitiello MF
现在人们认识到，除了免疫球蛋白(Ig)，M和D同种类型的鱼也能引起粘膜IGT。在这项研究中，我们对具有商业价值的东方金枪鱼(太平洋蓝鳍金枪鱼)的Igτ和μ的全长进行了测序，这是首次在波斯形目的成员中对这两个Ig亚型进行分子分析。金枪鱼IgM和IGT均由四个恒定的(CH)结构域组成。我们克隆和测序了48个金枪鱼免疫球蛋白不同可变区基因重排，并根据70%的核苷酸同源性将VH基因序列分为四个VH基因片段家族。有3个VH基因家族同时被IgM和IGT使用，但只有一组被IgM使用。最有趣的是，μ和τ克隆似乎都使用相同的多样性(DH)片段，这与其他物种中所描述的不同，尽管它们有专门的IGT和IgM连接(JH)基因片段。我们用系统发育和组织表达分析补充了这一谱系研究。除了支持在这一重要水产养殖物种中开发体液疫苗外，这些数据还表明，对于某些鱼类中携带IGT和IgM/D的淋巴细胞谱系来说，DH-JH重组而不是VH-DH重组可能具有指导意义。
It is now appreciated that in addition to the immunoglobulin (Ig)M and D isotypes fish also make the mucosal IgT. In this study we sequenced the full length of Ig τ as well as μ in the commercially important Thunnus orientalis (Pacific bluefin tuna), the first molecular analysis of these two Ig isotypes in a member of the order Perciformes. Tuna IgM and IgT are each composed of four constant (CH) domains. We cloned and sequenced 48 different variable (VH) domain gene rearrangements of tuna immunoglobulins and grouped the VH gene sequences to four VH gene segment families based on 70% nucleotide identity. Three VH gene families were used by both IgM and IgT but one group was only found to be used by IgM. Most interestingly, both μ and τ clones appear to use the same diversity (DH) segment, unlike what has been described in other species, although they have dedicated IgT and IgM joining (JH) gene segments. We complemented this repertoire study with phylogenetic and tissue expression analysis. In addition to supporting the development of humoral vaccines in this important aquaculture species, these data suggest that the DH-JH recombination rather than the VH-DH recombination may be instructive for IgT versus IgM/D bearing lymphocyte lineages in some fish.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.4
作者：
Edholm, Eva-Stina;Bengten, Eva;Wilson, Melanie
通讯作者：
Wilson, Melanie
影响因子：
56.9
作者：
Block, BA;Dewar, H;Fudge, D
通讯作者：
Fudge, D
影响因子：
2.9
作者：
Gambon-Deza, Francisco;Sanchez-Espinel, Christian;Magadan-Mompo, Susana
通讯作者：
Magadan-Mompo, Susana