Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities.
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities.
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DOI：
10.1016/j.jgr.2019.11.007
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发表时间：
2021-01
影响因子：
6.3
通讯作者：
Lim KM
中科院分区：
文献类型：
--
作者：
Kim K;Jeong HI;Yang I;Nam SJ;Lim KM
人参提取物和人参发酵产物因其美白和抗皱作用而被广泛用作功能性化妆品成分。近年来，从植物内生真菌中分离得到的具有生物活性的代谢产物越来越受到人们的关注。然而，很少有人知道的生物活性代谢产物的真菌与人参迈耶。从人参根中分离到一株内生真菌Penicillium sp.SNF123，并从中分离纯化了Acremonidin E。在鼠黑色素瘤细胞系B16 F10、人黑色素瘤细胞系MNT-1和有色3D-人皮肤模型Melanoderm中测试了Acremonidin E对黑色素合成的影响。在α-黑素细胞刺激激素（α-MSH）刺激的B16 F10细胞中，Acremonidin E以最小的细胞毒性减少黑素生成。qRT-PCR分析表明，acremonidin E下调黑素生成基因，包括酪氨酸酶和酪氨酸酶相关蛋白1（TRP-1），而它们的酶活性不受影响。Acremonidin E的抗黑色素生成作用在MNT-1和色素3D人表皮皮肤模型Melanoderm中得到进一步证实。黑色素瘤的免疫组织学检查进一步证实了治疗组织中黑色素合成和黑素细胞活化的消退。这项研究表明，acremonidin E，一种来自人参内生真菌的生物活性代谢产物，可以通过下调酪氨酸酶来抑制黑色素的合成，从而阐明了与人参相关的微生物在化妆品成分中的潜在用途。
Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioactive metabolites of the fungi associated with Panax ginseng Meyer. An endophytic fungus, Penicillium sp. SNF123 was isolated from the root of P. ginseng, from which acremonidin E was purified. Acremonidin E was tested on melanin synthesis in the murine melanoma cell line B16F10, in the human melanoma cell line MNT-1, and in a pigmented 3D-human skin model, Melanoderm. Acremonidin E reduced melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 cells with minimal cytotoxicity. qRT–PCR analysis demonstrated that acremonidin E downregulated melanogenic genes, including tyrosinase and tyrosinase-related protein 1 (TRP-1), while their enzymatic activities were unaffected. The antimelanogenic effects of acremonidin E were further confirmed in MNT-1 and a pigmented 3D human epidermal skin model, Melanoderm. Immunohistological examination of the Melanoderm further confirmed the regression of both melanin synthesis and melanocyte activation in the treated tissue. This study demonstrates that acremonidin E, a bioactive metabolite derived from a fungal endophyte of P. ginseng, can inhibit melanin synthesis by downregulating tyrosinase, illuminating the potential utility of microorganisms associated with P. ginseng for cosmetic ingredients.
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