Decreased Insulin Secretion but Unchanged Glucose Homeostasis in Cadmium-Exposed Male C57BL/6 Mice
Decreased Insulin Secretion but Unchanged Glucose Homeostasis in Cadmium-Exposed Male C57BL/6 Mice
复制标题
镉暴露的雄性 C57BL/6 小鼠胰岛素分泌减少，但血糖稳态未改变
DOI：
10.1155/2019/8121834
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发表时间：
2019-06
影响因子：
2.9
通讯作者：
Zengli Zhang
中科院分区：
文献类型：
--
作者：
Xiaoyin Li;Mengyang Li;Jiming Xu;Xiang zhang;Wei Xiao;Zengli Zhang
镉（Cd）是一种众所周知的有毒金属元素，大量分布在环境中。Cd对大多数器官都有毒性。越来越多的证据表明，Cd暴露与胰岛功能障碍和糖尿病的发展有关，但这种联系仍存在争议。本研究旨在探讨慢性Cd暴露对雄性C57BL/6小鼠糖代谢的可能影响。小鼠腹腔注射CdCl2溶液（1 mg.kg−1），每周2次，连续24周。每周测量空腹血糖（FBG）水平和体重。24周后进行腹腔葡萄糖耐量试验（IPGTT）、腹腔胰岛素耐量试验（IPITT）和空腹血清胰岛素（FSI）水平试验。计算并分析胰岛素抵抗指数（HOMA- ir）和胰腺β细胞功能指数（HOMA-β）。采用实时荧光定量PCR法检测小鼠肝脏中胰岛素受体（IR）的表达。收集胰腺组织进行组织学检查。结果表明，Cd暴露小鼠的FBG、IPGTT、HOMA- ir和HOMA-β与对照小鼠相同。与对照组相比，cd暴露小鼠的平均空腹血清胰岛素水平、IPITT曲线下面积（AUC）和肝脏IR表达均显著降低。Cd引起胰岛萎缩，胰岛面积减小。结果表明，Cd暴露可降低雄性C57BL/6小鼠的胰岛素分泌并维持葡萄糖稳态，长期Cd暴露人群应监测胰腺功能。
Cadmium (Cd) is a well-known toxic metal element that is largely distributed in the environment. Cd causes toxicity to most organs. Accumulating evidence suggests that Cd exposure is associated with islet dysfunction and development of diabetes, but the association remains controversial. The aim of this study is to evaluate the possible effects of chronic Cd exposure on glucose metabolism in male C57BL/6 mice. Mice were intraperitoneally injected with CdCl2 solution (1 mg.kg−1) twice a week for 24 weeks. Fasting blood glucose (FBG) levels and body weights were measured weekly. After 24 weeks, the intraperitoneal glucose tolerance test (IPGTT), intraperitoneal insulin tolerance test (IPITT), and fasting serum insulin (FSI) level test were performed. The insulin resistance index (HOMA-IR) and pancreatic β cell function index (HOMA-β) were calculated and analyzed. The expression of insulin receptor (IR) in mouse liver was detected by real-time PCR. Pancreatic tissue was collected for histological examination. The results demonstrated that FBG, IPGTT, HOMA-IR, and HOMA-β were identical between Cd exposure and control mice. In contract, mean fasting serum insulin level, area under the curve (AUC) of IPITT, and IR expression in livers of Cd-exposed mice decreased significantly compared with control mice. Cd administration induced islet atrophy and decreased islet area. The results suggested that Cd exposure decreased insulin secretion and maintained glucose homeostasis in male C57BL/6 mice and that pancreatic functions should be monitored in populations chronically exposed to Cd.
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影响因子：
16.6
作者：
Nagarajan, Arvindhan;Petersen, Max C.;Nasiri, Ali R.;Butrico, Gina;Fung, Annie;Ruan, Hai-Bin;Kursawe, Romy;Caprio, Sonia;Thibodeau, Jacques;Bourgeois-Daigneault, Marie-Claude;Sun, Lisha;Gao, Guangping;Bhanot, Sanjay;Jurczak, Michael J.;Green, Michael R.;Shulman, Gerald I.;Wajapeyee, Narendra
通讯作者：
Wajapeyee, Narendra
DOI：
--
发表时间：
2007-08
期刊：
Biomedical and environmental sciences : BES
影响因子：
--
作者：
Li-jian Lei;T. Jin;Yuan-fen Zhou
通讯作者：
Li-jian Lei;T. Jin;Yuan-fen Zhou
影响因子：
4.2
作者：
Rewers M;Hyöty H;Lernmark Å;Hagopian W;She JX;Schatz D;Ziegler AG;Toppari J;Akolkar B;Krischer J;TEDDY Study Group
通讯作者：
TEDDY Study Group
影响因子：
3.9
作者：
Liu, Ruohan;Jia, Tiantian;Li, Shu
通讯作者：
Li, Shu
影响因子：
3.3
作者：
Alderete TL;Chen Z;Toledo-Corral CM;Contreras ZA;Kim JS;Habre R;Chatzi L;Bastain T;Breton CV;Gilliland FD
通讯作者：
Gilliland FD