RNAi knock-downs support roles for the mucin-like (AeIMUC1) gene and short-chain dehydrogenase/reductase (SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum.
RNAi knock-downs support roles for the mucin-like (AeIMUC1) gene and short-chain dehydrogenase/reductase (SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum.
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DOI：
10.1111/j.1365-2915.2011.00965.x
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发表时间：
2012-03
影响因子：
1.9
通讯作者：
Severson DW
中科院分区：
文献类型：
--
作者：
Berois M;Romero-Severson J;Severson DW
蚊子的中肠是疟原虫从受感染的脊椎动物中摄取血液后遇到的第一道屏障。以前的研究确定了埃及伊蚊（L.）粘蛋白样（AeIMUC 1）和短链脱氢酶/还原酶（SDR）基因作为中肠表达的候选基因，影响鸡疟原虫（Brumpt）感染的易感性。在这里，我们使用RNA干扰（RNAi）的双链RNA注射（dsRNA），以检查动合子的生存卵囊阶段后，个别基因敲低。在感染鸡类原杆菌前三天进行dsRNA基因敲除，并在感染后七天评价卵囊发育。与注射dsβ-gal对照的雌性动物相比，注射dsAeIMUC 1的雌性动物中发育至卵囊期的寄生虫平均数量显著减少52.3%，而感染dsSDR的雌性动物中发育至卵囊期的寄生虫平均数量显著减少36.5%。与注射dsβ-gal对照的雌性动物相比，注射dsAeIMUC 1和dsSDR的雌性动物的感染率显著降低;这表示未感染个体的数量分别增加了2倍和3倍。总的来说，这些结果表明AeIMUC 1和SDR在Ae中发挥作用。埃及伊蚊对鸡疫霉的媒介感受态。
The mosquito midgut represents the first barrier that the Plasmodium parasite encounters after blood ingestion from an infected vertebrate. Previous studies identified the Aedes aegypti (L.) mucin-like (AeIMUC1) and short-chain dehydrogenase/reductase (SDR) genes as midgut-expressed candidate genes influencing susceptibility to infection by Plasmodium gallinaceum (Brumpt). Here we used RNA inference (RNAi) by double-stranded RNA injections (dsRNA) to examine ookinete survival to the oocyst stage following individual gene knockdowns. dsRNA gene knockdowns were performed three days prior to P. gallinaceum infections and oocyst development was evaluated at seven days post infection. Mean numbers of parasites developing to the oocyst stage were significantly reduced by 52.3% among dsAeIMUC1 injected females and by 36.5% among dsSDR infected females compared to females injected with a dsβ-gal control. Prevalence of infection was significantly reduced among dsAeIMUC1 and dsSDR injected females compared to females injected with a dsβ-gal control; this represented a two and three fold increase in the number of uninfected individuals, respectively. Overall, these results suggest that both AeIMUC1 and SDR play a role in Ae. aegypti vector competence to P. gallinaceum.
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影响因子：
2.6
作者：
Rayms-Keller, A;McGaw, M;Beaty, BJ
通讯作者：
Beaty, BJ
影响因子：
8
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Kallberg, Y;Oppermann, U;Persson, B
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3.3
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3.8
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通讯作者：
McElwee, JJ