Molecular epidemiology of human sporotrichosis in Venezuela reveals high frequency of Sporothrix globosa.
Molecular epidemiology of human sporotrichosis in Venezuela reveals high frequency of Sporothrix globosa.
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DOI：
10.1186/s12879-015-0839-6
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发表时间：
2015-02-25
影响因子：
3.7
通讯作者：
Niño-Vega GA
中科院分区：
文献类型：
--
作者：
Camacho E;León-Navarro I;Rodríguez-Brito S;Mendoza M;Niño-Vega GA
孢子丝菌病是人类和其他哺乳动物的皮肤和皮下真菌疾病，已知由申克孢子丝菌物种复合体引起，其包括四个具有临床重要性的物种：S. brasiliensis、巴西黑刺蛾S. globosa，S. luriei和S.严格意义上的申克氏病其中，S。globosa和S.申基湾str.显示全球分布和全球频率的差异，作为疾病的病因。在美洲，只有三种：S。申基湾str.，S. brasiliensis（目前仅在巴西有报道）和S. globosa。在委内瑞拉，自1935年报道第一例孢子虫病以来，S.申克氏菌被认为是其唯一的病原体。在目前的工作中，对该国一个以上物种的存在进行了评估。通过表型关键特征和分子遗传学分析，我们重新检查了来自委内瑞拉不同地区的30个菌株，这些菌株属于委内瑞拉加拉加斯生物医学研究所和国家皮肤病参考中心的真菌保藏中心。所有分离株均在1973年至2013年期间收集，并保存在蒸馏水中。委内瑞拉的孢子虫病主要由S.申基湾str.（70%）。然而，该国相当大比例（30%）的孢子虫病病例可归因于S。globosa。种内基因型和临床表现之间的相关性提出。我们的数据表明，孢子虫病的各种临床形式可能与分离株的遗传多样性有关，并可能与以前报道的S。申克种复合体在委内瑞拉重新检查的病例中，发现球形孢子丝菌是30%孢子虫病的病原体，这是迄今为止美洲报告的该物种的最高频率。S.申基湾str.表明基于表型关键特征的物种区分可能是一项具有挑战性和不确定性的任务;应始终采用分子鉴定。
Sporotrichosis is a cutaneous and subcutaneous fungal disease of humans and other mammals, known to be caused by the Sporothrix schenckii species complex, which comprises four species of clinical importance: S. brasiliensis, S. globosa, S. luriei, and S. schenckii sensu stricto. Of them, S. globosa and S. schenckii s. str. show global distribution and differences in global frequency as causal agents of the disease. In the Americas, only three species are present: S. schenckii s. str., S. brasiliensis (so far, only reported in Brazil), and S. globosa. In Venezuela, since the first case of sporotrichosis reported in 1935, S. schenckii have been considered its unique etiological agent. In the present work, the presence of more than one species in the country was evaluated. By phenotypic key features and molecular phylogeny analyses, we re-examined 30 isolates from diverse Venezuelan regions belonging to the fungi collection of Instituto de Biomedicina, Caracas, Venezuela, and national reference center for skin diseases. All isolates were collected between 1973 and 2013, and maintained in distilled water. Sporotrichosis in Venezuela is mainly caused by S. schenckii s. str. (70%). However, a significant proportion (30%) of sporotrichosis cases in the country can be attributable to S. globosa. A correlation between intraspecific genotypes and clinical presentation is proposed. Our data suggest that sporotrichosis various clinical forms might be related to genetic diversity of isolates, and possibly, to diverse virulence profiles previously reported in the S. schenckii species complex. Sporothrix globosa was found to be the causative agent of 30% of sporotrichosis for the Venezuelan cases re-examined, the highest frequency of this species so far reported in the Americas. The high genetic variability presented by S. schenckii s. str. indicates that species distinction based on phenotypic key features could be a challenging and uncertain task; molecular identification should be always employed.
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影响因子：
9.4
作者：
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通讯作者：
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