Dissolved organic metabolite extraction from high‐salt media
Dissolved organic metabolite extraction from high‐salt media
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从高盐介质中提取溶解的有机代谢物
DOI：
10.1002/nbm.4797
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发表时间：
2022
影响因子：
2.9
通讯作者：
Edison, Arthur S.
中科院分区：
文献类型：
--
作者：
Holderman, Nicole R.;Ferrer‐González, Frank X.;Glushka, John;Moran, Mary Ann;Edison, Arthur S.
我们描述了开发核磁共振（NMR）样品制备方法的考虑因素和策略，该方法用于在浮游植物和海洋细菌的模型共培养系统中从高盐废培养基中提取低分子量代谢物。浮游植物负责地球上一半的碳固定和氧气生成。固定碳的很大一部分成为被称为溶解有机物（DOM）的小分子代谢物库的一部分，这些小分子代谢物库被浮游植物附近的海洋细菌吸收。由于海水化学、表型和物种组成的广泛人为转变，迫切需要阐明这些代谢交换。这些变化导致水温升高，海洋吸收的二氧化碳量增加，海洋微生物消耗能量。人们对浮游植物和相关海洋细菌之间发生的代谢物介导的结构化相互作用知之甚少，部分原因是在各种分析平台上研究高盐溶液的挑战。由于天然海水和海洋微生物培养基的盐含量较高，核磁共振分析存在问题。高盐浓度会降低射频线圈的性能，降低某些脉冲序列的效率，限制信噪比，并延长实验时间。本文描述的方法可以从小体积、高盐培养物中重复提取低分子量 DOM。它是阐明海洋微生物之间代谢通量并促进突变微生物遗传筛选的有前途的工具。
We describe considerations and strategies for developing a nuclear magnetic resonance (NMR) sample preparation method to extract low molecular weight metabolites from high‐salt spent media in a model coculture system of phytoplankton and marine bacteria. Phytoplankton perform half the carbon fixation and oxygen generation on Earth. A substantial fraction of fixed carbon becomes part of a metabolite pool of small molecules known as dissolved organic matter (DOM), which are taken up by marine bacteria proximate to phytoplankton. There is an urgent need to elucidate these metabolic exchanges due to widespread anthropogenic transformations on the chemical, phenotypic, and species composition of seawater. These changes are increasing water temperature and the amount of CO2absorbed by the ocean at energetic costs to marine microorganisms. Little is known about the metabolite‐mediated, structured interactions occurring between phytoplankton and associated marine bacteria, in part because of challenges in studying high‐salt solutions on various analytical platforms. NMR analysis is problematic due to the high‐salt content of both natural seawater and culture media for marine microbes. High‐salt concentration degrades the performance of the radio frequency coil, reduces the efficiency of some pulse sequences, limits signal‐to‐noise, and prolongs experimental time. The method described herein can reproducibly extract low molecular weight DOM from small‐volume, high‐salt cultures. It is a promising tool for elucidating metabolic flux between marine microorganisms and facilitates genetic screens of mutant microorganisms.
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使用切片机低温恒温器装置进行样品均质的新方法
影响因子：
3.9
作者：
E. Zelentsova;V. Yanshole;Y. Tsentalovich
通讯作者：
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影响因子：
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作者：
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DOI：
10.1038/s41396-020-00811-y
发表时间：
2021-03
期刊：
The ISME journal
影响因子：
--
作者：
Ferrer-González FX;Widner B;Holderman NR;Glushka J;Edison AS;Kujawinski EB;Moran MA
通讯作者：
Moran MA
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DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
S. A. Richards;J. Hollerton
通讯作者：
J. Hollerton
影响因子：
11
作者：
Landa, Marine;Burns, Andrew S.;Moran, Mary Ann
通讯作者：
Moran, Mary Ann