A novel method of sample homogenization with the use of a microtome-cryostat apparatus
A novel method of sample homogenization with the use of a microtome-cryostat apparatus
复制标题
使用切片机低温恒温器装置进行样品均质的新方法
作者：
E. Zelentsova;V. Yanshole;Y. Tsentalovich
定量代谢组学对样品制备提出了很高的要求，包括高度的代谢物提取和受控的样品重量。对于富含胶原蛋白的弹性组织，现有的样品均质化方法由于不完全均质化、样品材料损失或代谢物降解而难以满足这些要求。在此，提出了一种基于使用显微镜-低温恒温器装置的新方法。的低温切片机的方法的性能进行了比较与使用的涡流珠殴打所获得的结果。基于NMR的代谢组学分析表明，两种样品制备方法的提取效率和数据分散性相似。然而，在珠击过程中产生的热量会破坏热不稳定的化合物;此外，它可能会导致蛋白质水解，导致氨基酸水平的人为增加。样品均质化的低温切片机方法不会引起样品加热，并且它似乎是弹性组织的理想方法。
Quantitative metabolomics places high demands on sample preparation, including a high degree of metabolite extraction and controlled sample weight. In respect to elastic collagen-rich tissues, the existing methods of sample homogenization poorly fit these demands due to incomplete homogenization, sample material loss, or metabolite degradation. Herein, a novel method based on the use of a microtome-cryostat apparatus is proposed. The performance of the cryotome method is compared with the results obtained with the use of a vortex bead beating. NMR-based metabolomic analysis shows that the extraction efficiency and the data scattering for both methods of sample preparation are similar. However, the heat generation during the bead beating causes the destruction of thermally-unstable compounds; besides, it may cause protein hydrolysis, leading to an artificial increase in the amino acid level. The cryotome method of sample homogenization does not cause sample heating, and it seems to be ideal for elastic tissues.
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影响因子：
7.7
作者：
Markley JL;Brüschweiler R;Edison AS;Eghbalnia HR;Powers R;Raftery D;Wishart DS
通讯作者：
Wishart DS
DOI：
10.1016/j.pnmrs.2017.01.001
发表时间：
2017-05
影响因子：
6.1
作者：
Marshall DD;Powers R
通讯作者：
Powers R
影响因子：
7.4
作者：
Nagana Gowda GA;Raftery D
通讯作者：
Raftery D
影响因子：
--
作者：
Riekeberg E;Powers R
通讯作者：
Powers R