Recombinant dynein light intermediate chain of Haemonchus contortus affects the functions of goat immune cells in vitro
Recombinant dynein light intermediate chain of Haemonchus contortus affects the functions of goat immune cells in vitro
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重组捻转血矛线虫动力蛋白轻中间链对山羊免疫细胞功能的体外影响
DOI：
10.1007/s00436-022-07510-x
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发表时间：
2022-04
影响因子：
2
通讯作者：
Ruofeng Yan
中科院分区：
文献类型：
--
作者：
Kalibixiati Aimulajiang;Zhaohai Wen;Faiz Muhammad Kh;Ambreen Leghari;Lixin Xu;Xiaokai Song;Xiangrui Li;Ruofeng Yan
捻转血矛线虫动力蛋白轻中间链（HcLIC）是捻转血矛线虫的一种重要的排泄分泌蛋白，具有抗原性。然而，对其对宿主免疫细胞的免疫调节作用的了解仍然有限。本研究采用PCR方法扩增HcLIC基因，并将其克隆到原核表达载体pET 32a中。通过IPTG表达蛋白，并通过使用HisTrap™ FF柱的亲和层析纯化。HcLIC在H.采用免疫组化方法检测扭转妇女的扭转情况。免疫荧光法（IFA）检测rHcLIC与山羊外周血单个核细胞（PBMCs）的结合能力。此外，当山羊PBMC与rHcLIC蛋白共孵育时，HcLIC对细胞迁移和细胞凋亡的影响进行了评估。结果表明，rHcLIC在成虫的表皮组织中有表达。扭曲IFA证实HcLIC与山羊PBMC表面结合。此外，功能分析显示，rHcLIC和宿主免疫细胞之间的相互作用显着抑制细胞迁移，这表明寄生虫可能会减少细胞因子和趋化因子的产生，这些细胞因子和趋化因子是宿主免疫细胞向感染部位迁移的信号。此外，rHcLIC处理提高了细胞凋亡效率，这可能降低免疫细胞数量，从而下调宿主免疫力，使寄生虫能够在宿主内存活。这些结果表明，随着诱导凋亡的发生，细胞迁移能力的下降趋势沿着，可能是H.扭曲总的来说，这些发现增加了我们对HcLIC的理解，以及H。宿主-寄生虫相互作用期间的捻转免疫逃逸。
Haemonchus contortus dynein light intermediate chain (HcLIC), an essential excretory/secretory protein of Haemonchus contortus, has been shown to have antigenic features. Neverthless, understanding of its immunomodulatory roles on host immune cells remains limited. Herein, HcLIC gene was amplified by polymerase chain reaction (PCR) and cloned in prokaryotic expression vector pET32a. The protein was expressed by IPTG and purified by affinity chromatography using HisTrap™ FF column. The localization of HcLIC in adult H. contortus woms was detected by immunohistochemical analysis. Immunofluorescence assay (IFA) was carried out to test the binding ability of rHcLIC to goat peripheral blood mononuclear cells (PBMCs). Furthermore, the effects of HcLIC on cell migration and cell apoptosis were evaluated when goat PBMCs were co-incubated with rHcLIC protein. The results revealed that rHcLIC was expressed in the cuticle tissues of adult H. contortus. IFA confirmed the binding of HcLIC on the surface of goat PBMCs. Moreover, functional analysis revealed that the interaction between rHcLIC and host immune cells significantly suppressed cell migration, suggesting that parasite might lessen the production of cytokines and chemokines that signal the migration of host immune cells towards infection site. Moreover, rHcLIC treatment improved cell apoptosis efficiency which might lower the immune cells quantity and thereby downregulate host immunity, enabling parasite survival within host. These results suggested that decrease trend of migration along with induction of apoptosis might be an immunosuppressive strategy of H. contortus. Overall, these findings add to our understanding of HcLIC, and the mechanisms involved in H. contortus immune escape during host-parasite interaction.
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影响因子：
3.7
作者：
Gadahi JA;Wang S;Bo G;Ehsan M;Yan R;Song X;Xu L;Li X
通讯作者：
Li X
影响因子：
7.2
作者：
Barlan K;Gelfand VI
通讯作者：
Gelfand VI
趋化因子的作用和免疫细胞在寄生虫感染中的运输。
影响因子：
--
作者：
McGovern KE;Wilson EH
通讯作者：
Wilson EH
影响因子：
3.2
作者：
Qiangqiang Wang;Lingyan Wu;M. W. Hasan;Mingmin Lu;Wenjuan Wang;Ruofeng Yan;Lixin Xu;Xiaokai Song;Xiangrui Li
通讯作者：
Qiangqiang Wang;Lingyan Wu;M. W. Hasan;Mingmin Lu;Wenjuan Wang;Ruofeng Yan;Lixin Xu;Xiaokai Song;Xiangrui Li
影响因子：
2
作者：
N. Viseshakul;Wilanee Dechkhajorn;Surachet Benjathummarak;S. Nuamtanong;Y. Maneerat
通讯作者：
N. Viseshakul;Wilanee Dechkhajorn;Surachet Benjathummarak;S. Nuamtanong;Y. Maneerat