Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei
Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei
复制标题
甲壳动物高血糖激素直接调节凡纳滨对虾血细胞的免疫反应
DOI：
10.1016/j.fsi.2017.01.007
复制
发表时间：
2017-03
影响因子：
4.7
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Wang Lin;Chen Hao;Xu Jianchao;Xu Qingsong;Wang Mengqiang;Zhao Depeng;Wang Lingling;Song Linsheng
机体对入侵病原体的免疫应答是其生存的关键，而机体的免疫应答在很大程度上依赖于机体代谢的平衡。越来越多的证据表明，一些代谢激素，如胰岛素，可以直接调节免疫反应。甲壳动物高血糖激素（CHH）家族是蜕皮动物特有的一组肽类激素，参与葡萄糖代谢等生物学事件。本研究选择了分别具有一个和两个甲壳类神经激素结构域的凡纳滨对虾CHH家族的两个成员（命名为LvCHH I和LvCHH II），以研究它们在葡萄糖代谢和免疫反应中的调节作用。LvCHH I和LvCHH II在眼柄的窦腺和神经节层中均有表达，且在白色斑综合征病毒（WSSV）感染后被显著诱导。同时，对虾感染WSSV后12和24 h血淋巴中葡萄糖含量显著升高，而血细胞中葡萄糖含量在6 h下降，12 h又上升。随后通过注射重组蛋白（rLvCHH I和rLvCHH II）在体内进行rLvCHH的功能获得。注射rLvCHH Ⅰ后0.5 ~ 3 h血淋巴葡萄糖显著升高，注射rLvCHH Ⅱ后0.5h血淋巴葡萄糖下降，3 h后血淋巴葡萄糖升高。同时，rLvCHH I注射后3 h和6 h血细胞活性氧水平明显降低，而rLvCHH II注射组无明显变化。rLvCHH I和rLvCHH II在体外可与原代对虾血细胞膜结合，与血细胞孵育3 h后，SOD和LvRelish的表达增加。同时，rLvCHH Ⅰ和rLvCHH Ⅱ也能诱导抗菌肽crustin和penaeidin-4的表达。这些结果表明，LvCHH家族除了参与葡萄糖代谢外，还可以直接调节宿主的免疫应答，为无脊椎动物代谢激素的免疫调节作用提供了新的认识。
A robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimpLitopenaeus vannameiwith one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response. LvCHH I and LvCHH II were both expressed in the sinus gland and lamina ganglionalis of eyestalks and were significantly induced after white spot syndrome virus (WSSV) infection. Meanwhile, significant increases of hemolymph glucose levels were observed in shrimp at 12 and 24 h after WSSV infection while the glucose inside the hemocytes decreased at 6 h and then increased at 12 h. Gain-of-function of rLvCHHs was subsequently conductedin vivoby injecting the recombinant proteins (rLvCHH I and rLvCHH II). The hemolymph glucose increased significantly from 0.5 h to 3 h after the shrimps received an injection of rLvCHH I, while it decreased at 0.5 h and increased afterward at 3 h post rLvCHH II injection. At the meantime, significant decreases of reactive oxygen species level in hemocytes were observed at 3 h and 6 h post rLvCHH I injection, while it remained unchanged in rLvCHH II injection group. rLvCHH I and rLvCHH II could bind to the cytomembrane of primary shrimp hemocytesin vitro, and the expressions of superoxide dismutase and LvRelish increased when the hemocytes were incubated with rLvCHH I for 3 h. Meanwhile, the expression of antimicrobial peptides, crustin and penaeidin-4, were also induced by rLvCHH I and rLvCHH II. These results demonstrated that host immune response, in addition to glucose metabolism, could be directly modulated by LvCHH family, and the present study provided new insights into the immunomodulation role of metabolic hormones in invertebrate.
登录
查看更多内容
影响因子：
4.7
作者：
Huang, Zih-Jhan;Kang, Shih-Ting;Chen, Li-Li
通讯作者：
Chen, Li-Li
影响因子：
4.5
作者：
Lago-Leston, Asuncion;Ponce, Elizabeth;Munoz, Ma. Enriqueta
通讯作者：
Munoz, Ma. Enriqueta
DOI：
10.1210/jcem.84.9.6104
发表时间：
1999-09
期刊：
The Journal of Clinical Endocrinology and Metabolism
影响因子：
--
作者：
A. Aljada;H. Ghanim;E. Assian;P. Mohanty;Wael Hamouda;R. Garg;P. Dandona
通讯作者：
A. Aljada;H. Ghanim;E. Assian;P. Mohanty;Wael Hamouda;R. Garg;P. Dandona
影响因子：
9.8
作者：
Bajgar A;Kucerova K;Jonatova L;Tomcala A;Schneedorferova I;Okrouhlik J;Dolezal T
通讯作者：
Dolezal T
影响因子：
4.7
作者：
R. Sun;L. Qiu;Feng Yue;Lingling Wang;Rui Liu;Zhi Zhou;Huan Zhang;L. Song
通讯作者：
R. Sun;L. Qiu;Feng Yue;Lingling Wang;Rui Liu;Zhi Zhou;Huan Zhang;L. Song