Transepithelial Anti-Neuroblastoma Response to Kale among Four Vegetable Juices Using In Vitro Model Co-Culture System.
Transepithelial Anti-Neuroblastoma Response to Kale among Four Vegetable Juices Using In Vitro Model Co-Culture System.
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DOI：
10.3390/nu13020488
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发表时间：
2021-02-02
期刊：
影响因子：
5.9
通讯作者：
Sengupta B
中科院分区：
文献类型：
--
作者：
Piletz JE;Mao Y;Roy D;Qizilbash B;Nkamssi E;Weir E;Graham J;Emmanuel M;Iqbal S;Brue K;Sengupta B
榨汁蔬菜被认为是一种抗癌治疗。基于不同植物化学物质的生物利用度，存在对抗癌绿色植物的排序（羽衣甘蓝>莴苣>莴苣>菠菜）的支持，也被一些有害分子（即，草酸钙）。我们开发了一种新的体外经上皮抗神经母细胞瘤模型系统。果汁在小肠中稀释一次。将它们应用于分裂的SH-SY 5 Y神经母细胞瘤细胞顶部的顶端Caco-2Bbe 1细胞，并利用汁液光谱法考虑跨上皮电阻（TEER）和细胞生长的变化。首先在单一培养中研究，羽衣甘蓝和菠菜是对SH-SY 5 Y最具细胞抑制作用的汁液，莴苣没有显示出效果，并且高（4.2%）菠菜具有细胞毒性。在共培养中，高浓度（4.2%）羽衣甘蓝抑制神经母细胞瘤生长的速度最快（3天）。到了第五天，甘蓝和羽衣甘蓝同样健壮。Letterium在5天内显示出较小的抗增殖作用，菠菜仍具有细胞毒性。菠菜的细胞毒性与草酸盐的主要红外波段相对应。羽衣甘蓝汁诱导SH-SY 5 Y细胞活性氧自由基和S期细胞周期阻滞。羽衣甘蓝和羽衣甘蓝的优势在上皮上也很明显，因为提高TEER水平被认为是健康的。羽衣甘蓝的独特功能对应于一个主要的荧光峰，共洗脱与山奈酚在高效液相色谱。由于抗癌等级顺序得到支持，该模型似乎验证了筛选抗癌果汁。
Juicing vegetables is thought to be an anticancer treatment. Support exists for a rank order of anticancer greens (kale > dandelion > lettuce > spinach) based on degrees of bioavailability of different phytochemicals, also offset by some noxious molecules (i.e., calcium-oxalate). We developed a new in vitro transepithelial anti-neuroblastoma model system. The juices were diluted as predicted once in the small intestine. They were applied to apical Caco-2Bbe1 cells atop dividing SH-SY5Y neuroblastoma cells, and changes in transepithelial electrical resistance (TEER) and cell growth were considered with juice spectroscopies. Studied first in monoculture, kale and dandelion were the most cytostatic juices on SH-SY5Ys, lettuce showed no effect, and high (4.2%) spinach was cytotoxic. In co-culture, high (4.2%) kale was quickest (three days) to inhibit neuroblastoma growth. By five days, dandelion and kale were equally robust. Lettuce showed small anti-proliferative effects at five days and spinach remained cytotoxic. Spinach’s cytotoxicity corresponded with major infrared bands indicative of oxalate. Kale juice uniquely induced reactive oxygen species and S-phase cell cycle arrest in SH-SY5Y. The superiority of kale and dandelion was also apparent on the epithelium, because raising TEER levels is considered healthy. Kale’s unique features corresponded with a major fluorescent peak that co-eluted with kaempferol during high performance liquid chromatography. Because the anticancer rank order was upheld, the model appears validated for screening anticancer juices.
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影响因子：
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作者：
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