Intermediate-sized filaments of human endothelial cells.
Intermediate-sized filaments of human endothelial cells.
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DOI：
10.1083/jcb.81.3.570
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发表时间：
1979-06
期刊：
影响因子：
--
通讯作者：
Weber K
中科院分区：
文献类型：
--
作者：
Franke WW;Schmid E;Osborn M;Weber K
人内皮细胞的特点是从unrestrictedcord平行的电子显微镜和间接免疫荧光显微镜使用特异性抗体对不同类别的中间大小的细丝。这些细胞中典型的中等大小的细丝的强烈发展的、松散的束不被针对前角蛋白的抗体或针对平滑肌结蛋白的抗体装饰。然而，它们被针对鼠“波形蛋白”的抗体强烈修饰，即，57，000 mol wt多肽，其是在间充质来源的各种细胞中占优势的中等大小细丝的主要蛋白质。细胞骨架制剂大大丰富的中等大小的细丝显示与鼠波形蛋白共迁移的多肽带的富集。这表明在人类内皮细胞中丰富的中等大小的细丝主要是波形蛋白类型，并且可以通过它们与啮齿动物的波形蛋白的交叉反应来证明。这些数据也加强了几个亚类的中等大小的细丝，这可以区分免疫程序的证据。
Human endothelial cells prepared from unbilical cords are characterized in parallel by electron microscopy and indirect immunofluorescence microscopy using specific antibodies against different classes of intermediate-sized filaments. The strongly developed, loose bundles of intermediate-sized filaments typically found in these cells are not decorated by antibodies against prekeratin or antibodies against smooth muscle desmin. They are, however, strongly decorated by antibodies directed against murine "vimentin," i.e., the 57,000 mol wt polypeptide which is the major protein of the intermediate-sized filaments predominant in various cells of mesenchymal origin. Cytoskeletal preparations greatly enriched in intermediate-sized filaments show the enrichment of a polypeptide band comigrating with murine vimentin. This shows that the intermediate-sized filaments that are abundant in human endothelial cells are predominantly of the vimentin type and can be demonstrated by their cross-reaction with the vimentin of rodents. These data also strengthen the evidence for several subclasses of intermediate-sized filaments, which can be distinguished by immunological procedures.
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影响因子：
64.5
作者：
HYNES, RO;DESTREE, AT
通讯作者：
DESTREE, AT
影响因子：
7.8
作者：
DROCHMANS, P;FREUDENSTEIN, C;FRANKE, WW
通讯作者：
FRANKE, WW
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
DOI：
10.1016/0309-1651(78)90068-1
发表时间：
1978-01-01
期刊：
CELL BIOLOGY INTERNATIONAL REPORTS
影响因子：
--
作者：
FREUDENSTEIN, C;FRANKE, WW;WEBER, K
通讯作者：
WEBER, K
影响因子：
64.5
作者：
GORDON, WE;BUSHNELL, A;BURRIDGE, K
通讯作者：
BURRIDGE, K