Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function
Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function
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不同培养方法改变羊水MSCs中CD105表达而不影响分化能力或免疫功能
DOI：
10.1111/jcmm.15081
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发表时间：
2020-03
影响因子：
5.3
通讯作者：
Sun Xiaofang
中科院分区：
文献类型：
--
作者：
Wang Ding;Liu Nengqing;Xie Yingjun;Song Bing;Kong Shu;Sun Xiaofang
骨髓间充质干细胞是一种存在于不同组织中的培养细胞，作为生理和病理过程的诱导剂或调节剂。在这里，我们从羊水中获得的MSC，并比较其分化能力和PHA激活的PBMC的免疫抑制效果与脐带分离的MSC。分离羊水MSC，并在商业AFC培养基和经典MSC培养基上进行培养，并使用集落的数量和大小来评估原代和传代培养的差异。在传代培养的细胞中测量增殖率、群体倍增时间、细胞形态、细胞表面标志物和mRNA表达。此外，与脐带间充质干细胞进行比较研究，以评估PHA刺激的PBMC的分化能力和免疫抑制作用。羊水间充质干细胞经三种方法分离、扩增后，几乎具有脐带间充质干细胞的所有特征。与脐带MSCs相比，羊水MSCs具有更强的成骨、成软骨分化能力和更强的抑制PHA激活的PBMC分裂的免疫抑制作用。用商业AFC培养基培养产生了最高百分比的CD105表达，并且在原代细胞分离、细胞源特异性标记保留和细胞增殖方面显示出一些优势。结果表明，羊水间充质干细胞较脐带间充质干细胞具有一定的优势，不同的培养液可引起细胞增殖、细胞表面标志物和细胞形态的改变，但与分化能力和免疫效应的差异无关。
MSCs are kind of cultured cells that reside in different tissues as inducers or regulators of physiological and pathological processes. Here, we derived MSCs from amniotic fluid and compared their differentiation ability and immunosuppression effect on PHA-activated PBMC with those of MSCs isolated from umbilical cords. Amniotic fluid MSCs were isolated and cultured on commercial AFC medium and classic MSC medium, and the number and size of colonies were used to evaluate differences in primary and passaged culture. Rate of proliferation, population doubling time, cell morphology, cell surface markers and mRNA expression were measured in subcultured cells. Furthermore, a comparative study was performed with umbilical cord MSCs to assess the ability of differentiation and immunosuppressive effect of PHA-stimulated PBMCs. Amniotic fluid MSCs were isolated and expanded by three methods, and exhibited nearly all the characteristics of umbilical cord MSCs. Compared with umbilical cord MSCs, amniotic fluid MSCs had an enhanced osteogenic and chrondrogenic differentiation capability, and stronger immunosuppression effect of inhibition of PHA-activated PBMC division. Culture with commercial AFCs medium yielded the highest percentage of CD105 expression and showed some advantages in primary cell isolation, cell source-specific marker retention and cell proliferation. We demonstrated that amniotic fluid MSCs exhibited some advantages over umbilical cord MSCs, and different culture media caused cell proliferation, cell surface marker and cell morphology change, but were not associated with varying differentiation capability and immune effects.
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作者：
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作者：
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DOI：
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发表时间：
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影响因子：
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