Chronic changes in the articular cartilage and meniscus following traumatic impact to the lapine knee.
Chronic changes in the articular cartilage and meniscus following traumatic impact to the lapine knee.
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DOI：
10.1016/j.jbiomech.2014.11.038
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发表时间：
2015-01-21
影响因子：
2.4
通讯作者：
Donahue, Tammy L. Haut
中科院分区：
文献类型：
--
作者：
Fischenich, Kristine M.;Button, Keith D.;Coatney, Garrett A.;Fajardo, Ryan S.;Leikert, Kevin M.;Haut, Roger C.;Donahue, Tammy L. Haut
本研究的目的是通过单次胫股压缩撞击诱导前交叉韧带（ACL）和胫骨损伤，以记录撞击后关节软骨和胫骨的变化。Flemish Giant兔的胫股关节受到单次钝性撞击，导致ACL断裂并产生急性踝关节损伤。在人道处死前，允许动物不受限制地在笼中活动12周。关节软骨的印度墨水分析显示，与对照组相比，受影响的胫骨（p=0.018）和股骨（p<0.0001）的表面损伤程度更高。慢性腰椎损伤最常见于内侧中央和内侧后部区域。力学测试显示，与对照组相比，受影响关节的胫骨平台软骨厚度总体增加19.4%（p=0.026），胫骨平台渗透性增加34.8%（p=0.054），股骨髁渗透性增加40.8%（p=0.029），股骨髁基质模量降低20.1%（p=0.012）。与对照组相比，外侧和内侧髁突的瞬时模量和平衡模量均降低（p<0.02）。组织学分析显示，股骨内侧的裂缝显著增加（p = 0.036）。在半月板和软骨中，受影响肢体的GAG覆盖率显著降低。基于这些结果，很明显，未加注意的踝关节和ACL联合损伤导致损伤后12周该实验关节中软组织的显著变化。这些变化与膝关节中晚期PTOA的临床描述一致。
The objective of this study was to induce anterior cruciate ligament (ACL) and meniscal damage, via a single tibiofemoral compressive impact, in order to document articular cartilage and meniscal changes post impact. Tibiofemoral joints of Flemish Giant rabbits were subjected to a single blunt impact that ruptured the ACL and produced acute meniscal damage. Animals were allowed unrestricted cage activity for 12 weeks before euthanasia. India ink analysis of the articular cartilage revealed higher degrees of surface damage on the impacted tibias (p=0.018) and femurs (p<0.0001) compared to controls. Chronic meniscal damage was most prevalent in the medial central and medial posterior regions. Mechanical tests revealed an overall 19.4% increase in tibial plateau cartilage thickness (p=0.026), 34.8% increase in tibial plateau permeability (p=0.054), 40.8% increase in femoral condyle permeability (p=0.029), and 20.1% decrease in femoral condyle matrix modulus (p=0.012) in impacted joints compared to controls. Both the instantaneous and equilibrium moduli of the lateral and medial menisci were decreased compared to control (p<0.02). Histological analyses revealed significantly increased presence of fissures in the medial femur (p = 0.036). In both the meniscus and cartilage there was a significant decrease in GAG coverage for the impacted limbs. Based on these results it is clear that an unattended combined meniscal and ACL injury results in significant changes to the soft tissues in this experimental joint 12 weeks post injury. Such changes are consistent with a clinical description of mid to late stage PTOA of the knee.
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DOI：
10.1016/j.arthro.2012.03.015
发表时间：
2012-10-01
影响因子：
4.7
作者：
Brophy, Robert H.;Martinez, Mario;Silva, Matthew J.
通讯作者：
Silva, Matthew J.
DOI：
10.1115/1.4027468
发表时间：
2014-07-01
影响因子：
1.7
作者：
Fischenich, Kristine M.;Coatney, Garrett A.;Donahue, Tammy L. Haut
通讯作者：
Donahue, Tammy L. Haut
影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
影响因子：
4.8
作者：
Levine, Jason W.;Kiapour, Ata M.;Demetropoulos, Constantine K.
通讯作者：
Demetropoulos, Constantine K.
影响因子：
2.4
作者：
Ewers, BJ;Jayaraman, VM;Haut, RC
通讯作者：
Haut, RC