bak1-5 mutation uncouples tryptophan-dependent and independent postinvasive immune pathways triggered in Arabidopsis by multiple fungal pathogens
bak1-5 mutation uncouples tryptophan-dependent and independent postinvasive immune pathways triggered in Arabidopsis by multiple fungal pathogens
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bak1-5突变解开多种真菌病原体在拟南芥中触发的色氨酸依赖性和独立的侵袭后免疫途径
DOI：
10.1101/2020.04.26.052480
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yoshitaka Takano
中科院分区：
文献类型：
--
作者：
Ayumi Kosaka;Marta Pastorczyk;Mariola Pislewska-Bednarek;Takumi Nishiuchi;Haruka Suemoto;Atsushi Ishikawa;Henning Frerigmann;Masanori Kaido;Kazuyuki Mise;Pawel Bednarek;Yoshitaka Takano
拟南芥对非适应性半生物营养真菌热带炭疽菌（colletotrichum tropicalerer1）的强大非寄主抗性需要侵袭前依赖的pen2和侵袭后依赖的CYP71A12/ cyp71a13抗性，两者都依赖于色氨酸（Trp）代谢。在这里，我们报道了CYP71A12和CYP71A13在拟南芥对坏死性芸苔菌和适应性半营养性芸苔菌的侵袭后抗性中起关键作用。higginsianumfungi。代谢物分析表明，吲哚-3-羧酸衍生物（ICAs）和camalexin的产生是在病原体入侵时诱导的，而cyp79b2、cyp79b3和pen2、cyp71A12、cyp71a13植株的表型比较表明，ICAs对入侵后抗性的贡献是剂量依赖性的。我们还发现，由bak1 - 5突变引起的完整模式识别受体复合物的破坏显著降低了侵袭后对c的抵抗。tropicaleandA。表明模式识别通常有助于对具有不同感染策略的病原体的第二层防御。然而，bak1 - 5突变对病原体入侵引发的色氨酸代谢物积累没有可检测到的影响。除此之外，进一步的比较基因表达分析表明，病原体入侵在拟南芥中激活了(i)bak1 - 5不敏感的trp代谢，导致抗菌次级代谢物的产生，以及（ii） abak1 - 5敏感的免疫途径，激活抗菌蛋白的表达。
Robust nonhost resistance ofArabidopsis thalianaagainst the nonadapted hemibiotrophic fungusColletotrichum tropicalerequires PEN2-dependent preinvasive and CYP71A12/CYP71A13-dependent postinvasive resistance, which both rely on tryptophan (Trp) metabolism. Here we report that CYP71A12 and CYP71A13 are critical for Arabidopsis’ postinvasive resistance toward both the necrotrophicAlternaria brassicicolaand the adapted hemibiotrophicC. higginsianumfungi. Metabolite analyses suggest that the production of indole-3-carboxylic acid derivatives (ICAs) and camalexin is induced upon pathogen invasion, while phenotypic comparison ofcyp79B2 cyp79B3andpen2 cyp71A12 cyp71A13plants indicates that the contribution of ICAs to postinvasive resistance is dose-dependent. We also found that the disruption of intact pattern recognition receptor complex caused bybak1–5mutation significantly reduced postinvasive resistance againstC. tropicaleandA. brassicicola, indicating that pattern recognition commonly contributes to this second defense-layer against pathogens with distinct infection strategies. However, thebak1–5mutation had no detectable effects on Trp-metabolite accumulation triggered by pathogen invasion. Together with this, further comparative gene expression analyses suggested that pathogen invasion in Arabidopsis activates (i)bak1–5insensitive Trp-metabolism that leads to antimicrobial secondary metabolites, and (ii) abak1–5sensitive immune pathway that activates the expression of antimicrobial proteins.
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