Comparing the development of cortex-wide gene expression patterns between two species in a common reference frame.
Comparing the development of cortex-wide gene expression patterns between two species in a common reference frame.
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DOI：
10.1073/pnas.2113896119
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发表时间：
2022-10-11
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
我们创建了新的软件工具，用于在三维空间比较不同物种大脑之间的基因表达空间模式。使用这些工具，我们展示了如何在发育中的物种之间保持皮层范围的mRNA表达模式，重点是两个涉及定义皮质区域的基因。具体地说，Id2的表达模式是在小鼠和田鼠之间高度保守的逐层序列中发展起来的，而RZRβ在小鼠中的表达发展得更缓慢，特别是在初级视觉区域，其大小在出生后第一周翻了一番。这些发现证明了我们的开源软件的实用性，使来自许多领域的科学家能够量化基因在不同发育阶段和不同物种之间表达的地点和时间。测序技术的进步使基因表达的比较研究成为了解进化和发育过程的当前焦点。然而，由于缺乏可靠的方法，对基因空间表达的洞察一直受到限制。为了克服这一障碍，我们开发了量化和比较物种内和物种之间基因表达的组织范围空间模式的方法和软件工具。在这里，我们比较了RZRβ和Id2mRNA在小鼠和田鼠出生后早期发育过程中皮质范围的表达。我们发现，RZRβ在新皮质第四层的表达模式在物种之间高度保守，但在田鼠中发展迅速，在小鼠中发展得更缓慢，小鼠出生后第一周显示出推测的主要视觉区域的相对大小显著扩大。相比之下，Id2的表达模式出现在两个物种之间一致的动态和特定于层的序列中。我们认为，新皮质模式发展的这些差异反映了自上一个共同祖先以来3500万年来大脑、身体和感觉系统的独立进化。
We created new software tools for comparing spatial patterns of gene expression between the brains of different species in three dimensions. Using these tools, we show how cortex-wide patterns of mRNA expression are conserved between developing species, focusing on two genes involved in defining cortical fields. Specifically, Id2 expression patterns developed in a layer-by-layer sequence that was highly conserved between mice and voles, whereas RZRβ expression developed more gradually in mice, particularly in the primary visual area, which doubled in size over the first postnatal week. These findings demonstrate the usefulness of our open-source software for allowing scientists from many fields to quantify where and when genes are expressed across development and between species. Advances in sequencing techniques have made comparative studies of gene expression a current focus for understanding evolutionary and developmental processes. However, insights into the spatial expression of genes have been limited by a lack of robust methodology. To overcome this obstacle, we developed methods and software tools for quantifying and comparing tissue-wide spatial patterns of gene expression within and between species. Here, we compare cortex-wide expression of RZRβ and Id2 mRNA across early postnatal development in mice and voles. We show that patterns of RZRβ expression in neocortical layer 4 are highly conserved between species but develop rapidly in voles and much more gradually in mice, who show a marked expansion in the relative size of the putative primary visual area across the first postnatal week. Patterns of Id2 expression, by contrast, emerge in a dynamic and layer-specific sequence that is consistent between the two species. We suggest that these differences in the development of neocortical patterning reflect the independent evolution of brains, bodies, and sensory systems in the 35 million years since their last common ancestor.
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影响因子：
16.2
作者：
Cadwell, Cathryn R.;Bhaduri, Aparna;Mostajo-Radji, Mohammed A.;Keefe, Matthew G.;Nowakowski, Tomasz J.
通讯作者：
Nowakowski, Tomasz J.
影响因子：
3
作者：
Beery AK;Christensen JD;Lee NS;Blandino KL
通讯作者：
Blandino KL
影响因子：
2.5
作者：
Campi, Katharine L.;Karlen, Sarah J.;Krubitzer, Leah
通讯作者：
Krubitzer, Leah
影响因子：
3.7
作者：
Campi, Katharine L.;Bales, Karen L.;Krubitzer, Leah
通讯作者：
Krubitzer, Leah
影响因子：
1.7
作者：
Krubitzer, Leah;Campi, Katharine L.;Cooke, Dylan F.
通讯作者：
Cooke, Dylan F.