Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses.
Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses.
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DOI：
10.1186/s12985-018-0979-6
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发表时间：
2018-04-10
期刊：
影响因子：
4.8
通讯作者：
Chang KC
中科院分区：
文献类型：
--
作者：
Slater T;Eckerle I;Chang KC
随着最近在蝙蝠中发现新的H17 N10和H18 N11流感病毒RNA，并报道了一种自由放养的蝙蝠中禽流感H9血清转化的高频率，需要解决的一个重要问题是蝙蝠对传统禽流感和人类流感A病毒的易感程度。为此，分别用两种禽流感病毒和两种人流感病毒感染三种蝙蝠（Eidolon helvum，Eidolia perspicillata和Tadarida brasiliensis）的肺上皮细胞，以确定其相对宿主的先天免疫抵抗力。所有三种蝙蝠细胞对所有四种流感病毒的抗性均高于阳性对照Madin-Darby犬肾（MDCK）细胞。TB 1-Lu细胞缺乏唾液酸α 2，6-Gal受体，并且在三种蝙蝠中最耐药。有趣的是，与人类病毒相比，鸟类病毒在所有三种蝙蝠细胞中的复制能力相对更强，这表明蝙蝠可能在禽流感病毒的生态学中发挥作用。蝙蝠细胞中JAK-STAT途径的化学抑制对病毒产生没有影响，表明I型干扰素信号传导不是抵抗流感病毒感染的主要因素。虽然所有三种蝙蝠细胞对流感病毒感染的抵抗力都比对照MDCK细胞强，但它们对禽流感病毒的耐受性比对人流感病毒的耐受性强，这表明蝙蝠可能在禽流感病毒的生态学中起着重要作用。
With the recent discovery of novel H17N10 and H18N11 influenza viral RNA in bats and report on high frequency of avian H9 seroconversion in a species of free ranging bats, an important issue to address is the extent bats are susceptible to conventional avian and human influenza A viruses. To this end, three bat species (Eidolon helvum, Carollia perspicillata and Tadarida brasiliensis) of lung epithelial cells were separately infected with two avian and two human influenza viruses to determine their relative host innate immune resistance to infection. All three species of bat cells were more resistant than positive control Madin-Darby canine kidney (MDCK) cells to all four influenza viruses. TB1-Lu cells lacked sialic acid α2,6-Gal receptors and were most resistant among the three bat species. Interestingly, avian viruses were relatively more replication permissive in all three bat species of cells than with the use of human viruses which suggest that bats could potentially play a role in the ecology of avian influenza viruses. Chemical inhibition of the JAK-STAT pathway in bat cells had no effect on virus production suggesting that type I interferon signalling is not a major factor in resisting influenza virus infection. Although all three species of bat cells are relatively more resistant to influenza virus infection than control MDCK cells, they are more permissive to avian than human viruses which suggest that bats could have a contributory role in the ecology of avian influenza viruses.
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DOI：
10.1128/aem.16.4.588-594.1968
发表时间：
1968-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
GAUSH, CR;SMITH, TF
通讯作者：
SMITH, TF
影响因子：
4.8
作者：
Bernasconi, D;Amici, C;Santoro, MG
通讯作者：
Santoro, MG
影响因子：
64.8
作者：
WEIS, W;BROWN, JH;WILEY, DC
通讯作者：
WILEY, DC
影响因子：
3.7
作者：
Eckerle I;Ehlen L;Kallies R;Wollny R;Corman VM;Cottontail VM;Tschapka M;Oppong S;Drosten C;Müller MA
通讯作者：
Müller MA
影响因子：
4
作者：
Kuchipudi, Suresh V.;Dunham, Stephen P.;Nelli, Rahul;White, Gavin A.;Coward, Vivien J.;Slomka, Marek J.;Brown, Ian H.;Chang, Kin Chow
通讯作者：
Chang, Kin Chow