Bat airway epithelial cells: a novel tool for the study of zoonotic viruses.
Bat airway epithelial cells: a novel tool for the study of zoonotic viruses.
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DOI：
10.1371/journal.pone.0084679
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Müller MA
中科院分区：
文献类型：
--
作者：
Eckerle I;Ehlen L;Kallies R;Wollny R;Corman VM;Cottontail VM;Tschapka M;Oppong S;Drosten C;Müller MA
蝙蝠越来越被认为是重要的人畜共患病病毒的宿主。然而，到目前为止，在细胞培养中分离蝙蝠传播的病毒的许多尝试都没有成功。此外，对水库寄主物种的实验研究由于饲养这些物种的难度而受到限制。呼吸道上皮在空气传播过程中发挥着核心作用，因为它是病毒颗粒遇到的第一个组织。虽然最近已经建立了几个蝙蝠细胞系，但到目前为止还没有特征良好的、选择性培养的呼吸道上皮细胞。在标准化条件下，通过器官外植体的细胞生长和无血清原代细胞培养，成功地从新鲜和冷冻的器官标本中培养出两个重要亚目蝙蝠亚目蝙蝠的原代细胞和永生化细胞系。细胞被永生化以产生永久的细胞系。对细胞的上皮性进行鉴定，如细胞角蛋白和紧密连接蛋白的表达，以及对裂谷热病毒和印第安纳州水泡性口炎病毒的病毒感染的敏感性。这些细胞可作为研究蝙蝠传播病毒的合适模型，并可作为病毒感染人呼吸道上皮细胞的补充细胞培养模型。
Bats have been increasingly recognized as reservoir of important zoonotic viruses. However, until now many attempts to isolate bat-borne viruses in cell culture have been unsuccessful. Further, experimental studies on reservoir host species have been limited by the difficulty of rearing these species. The epithelium of the respiratory tract plays a central role during airborne transmission, as it is the first tissue encountered by viral particles. Although several cell lines from bats were established recently, no well-characterized, selectively cultured airway epithelial cells were available so far. Here, primary cells and immortalized cell lines from bats of the two important suborders Yangochiroptera and Yinpterochiroptera, Carollia perspicillata (Seba's short-tailed bat) and Eidolon helvum (Straw-colored fruit bat), were successfully cultured under standardized conditions from both fresh and frozen organ specimens by cell outgrowth of organ explants and by the use of serum-free primary cell culture medium. Cells were immortalized to generate permanent cell lines. Cells were characterized for their epithelial properties such as expression of cytokeratin and tight junctions proteins and permissiveness for viral infection with Rift-Valley fever virus and vesicular stomatitis virus Indiana. These cells can serve as suitable models for the study of bat-borne viruses and complement cell culture models for virus infection in human airway epithelial cells.
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影响因子：
16.6
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影响因子：
3.7
作者：
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通讯作者：
Guan, Yi
影响因子：
3.7
作者：
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通讯作者：
Cunningham, Andrew A.