Rapid death of duck cells infected with influenza: a potential mechanism for host resistance to H5N1.
Rapid death of duck cells infected with influenza: a potential mechanism for host resistance to H5N1.
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DOI：
10.1038/icb.2011.17
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发表时间：
2012-01
影响因子：
4
通讯作者：
Chang, Kin Chow
中科院分区：
文献类型：
--
作者：
Kuchipudi, Suresh V.;Dunham, Stephen P.;Nelli, Rahul;White, Gavin A.;Coward, Vivien J.;Slomka, Marek J.;Brown, Ian H.;Chang, Kin Chow
水生鸟类是大多数甲型流感亚型的天然宿主，也是可能导致人类大流行病、致命的人畜共患病或家禽中毁灭性流行病的新型病毒的来源。众所周知，水禽在感染甲型流感后通常表现出很少的临床症状，相反，陆生家禽如鸡在感染高致病性禽流感后可能发展为严重疾病并迅速死亡。本研究检测了来自抗性（鸭）和敏感（鸡）禽类宿主的原代细胞对流感感染的细胞应答。有趣的是，我们观察到鸭细胞在感染低致病性禽H2 N3、经典猪H1N1和“经典”高致病性H5 N1病毒后发生快速细胞死亡。死亡细胞表现出凋亡的形态学特征，DNA片段化增加和caspase 3/7活化。感染鸡细胞后，细胞死亡发生不那么迅速，伴随着DNA片段化和半胱天冬酶激活减少。与鸡细胞相比，鸭细胞产生类似水平的病毒RNA，但感染性较低。这种快速的细胞死亡没有观察到在鸭细胞感染当代欧亚血统H5 N1致命的鸭。诱导鸭细胞快速死亡可能是宿主对甲型流感病毒产生抗性的机制的一部分，这种反应的丧失导致对H5 N1新出现毒株的易感性增加。这些研究提供了新的见解，应有助于解决高致病性和人畜共患禽流感的宿主-病原体关系的长期之谜。
Aquatic birds are the natural reservoir for most subtypes of influenza A, and a source of novel viruses with the potential to cause human pandemics, fatal zoonotic disease or devastating epizootics in poultry. It is well recognised that waterfowl typically show few clinical signs following influenza A infection, in contrast, terrestrial poultry such as chickens may develop severe disease with rapid death following infection with highly pathogenic avian influenza. This study examined the cellular response to influenza infection in primary cells derived from resistant (duck) and susceptible (chicken) avian hosts. Paradoxically, we observed that duck cells underwent rapid cell death following infection with low pathogenic avian H2N3, classical swine H1N1 and ‘classical' highly pathogenic H5N1 viruses. Dying cells showed morphological features of apoptosis, increased DNA fragmentation and activation of caspase 3/7. Following infection of chicken cells, cell death occurred less rapidly, accompanied by reduced DNA fragmentation and caspase activation. Duck cells produced similar levels of viral RNA but less infectious virus, in comparison with chicken cells. Such rapid cell death was not observed in duck cells infected with a contemporary Eurasian lineage H5N1 fatal to ducks. The induction of rapid death in duck cells may be part of a mechanism of host resistance to influenza A, with the loss of this response leading to increased susceptibility to emergent strains of H5N1. These studies provide novel insights that should help resolve the long-standing enigma of host–pathogen relationships for highly pathogenic and zoonotic avian influenza.
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影响因子：
2.4
作者：
ALEXANDER, DJ;ALLAN, WH;PARSONS, G
通讯作者：
PARSONS, G
影响因子：
5.4
作者：
McLean, Jeffrey E.;Datan, Emmanuel;Zakeri, Zahra F.
通讯作者：
Zakeri, Zahra F.
影响因子：
2.8
作者：
Antarasena, Chongmas;Sirimujalin, Rungtiva;Prommuang, Praison
通讯作者：
Prommuang, Praison
人类禽流感（H5N1）感染的病理，分子生物学和发病机理。
影响因子：
6
作者：
Korteweg, Christine;Gu, Jiang
通讯作者：
Gu, Jiang
DOI：
10.1038/nrmicro2071
发表时间：
2009-02
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
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