Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model.
Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model.
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大鼠骨质疏松模型椎体骨髓灌注半定量和定量动态对比增强MRI评估的比较
DOI：
10.1186/s12891-017-1800-1
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发表时间：
2017-11-14
影响因子：
2.3
通讯作者：
Tang G
中科院分区：
文献类型：
--
作者：
Zhu J;Xiong Z;Zhang J;Qiu Y;Hua T;Tang G
背景本研究旨在探讨半定量和定量动态对比增强磁共振成像（DCE-MRI）在评价骨质疏松大鼠模型骨髓灌注纵向变化中的技术可行性，使用通过微型计算机断层扫描测量的骨矿物质密度（BMD）方法将50只大鼠随机分为对照组（n=25）和卵巢切除组（OVX组，n=25）。在基线和术后3、6、9和12周对腰椎进行半定量和定量DCE-MRI、micro-CT和组织病理学检查。使用独立样本检验比较两组在每个时间点的半定量DCE-MRI参数（最大增强，Emax）、定量DCE-MRI参数（体积传递常数，Ktranss;间质体积，Ve;和外排速率常数，Kep）、微CT参数（BMD）和组织病理学参数（微血管密度，MVD）方面的差异。通过Bonferroni多重比较检验评估各组中基线和其他时间点之间这些参数的差异。Pearson相关性分析被应用于评估DCE-MRI，micro-CT，和组织病理学参数之间的关系。ResultsIn OVX组，Emax值显着下降，与对照组相比，在第6周和第9周（p=0.003和0.004，分别）。从第3周开始，Ktranss值与对照组相比显著降低（p<0.05）。然而，仅在第9周时，Vevalues显著降低（p=0.032），两组之间未发现Kep差异。从第3周开始，OVX组骨密度值较对照组显著降低（p<0.05）。透射电子显微镜显示，从第3周开始，OVX组血管内皮细胞之间的间隙变紧，线粒体肿胀。仅在第12周时，OVX组的MVD值与对照组相比显著降低（p=0.023）。结论与半定量DCE-MRI相比，定量DCE-MRI参数Ktranss是一个更敏感、更准确的检测骨质增生早期灌注减少的指标。
BackgroundThis study aims to investigate the technical feasibility of semi-quantitative and quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the assessment of longitudinal changes of marrow perfusion in a rat osteoporosis model, using bone mineral density (BMD) measured by micro-computed tomography (micro-CT) and histopathology as the gold standards.MethodsFifty rats were randomly assigned to the control group (n=25) and ovariectomy (OVX) group whose bilateral ovaries were excised (n=25). Semi-quantitative and quantitative DCE-MRI, micro-CT, and histopathological examinations were performed on lumbar vertebrae at baseline and 3, 6, 9, and 12 weeks after operation. The differences between the two groups in terms of semi-quantitative DCE-MRI parameter (maximum enhancement, Emax), quantitative DCE-MRI parameters (volume transfer constant, Ktrans; interstitial volume, Ve; and efflux rate constant, Kep), micro-CT parameter (BMD), and histopathological parameter (microvessel density, MVD) were compared at each of the time points using an independent-samplettest. The differences in these parameters between baseline and other time points in each group were assessed via Bonferroni’s multiple comparison test. A Pearson correlation analysis was applied to assess the relationships between DCE-MRI, micro-CT, and histopathological parameters.ResultsIn the OVX group, the Emaxvalues decreased significantly compared with those of the control group at weeks 6 and 9 (p=0.003 and 0.004, respectively). The Ktransvalues decreased significantly compared with those of the control group from week 3 (p<0.05). However, the Vevalues decreased significantly only at week 9 (p=0.032), and no difference in the Kepwas found between two groups. The BMD values of the OVX group decreased significantly compared with those of the control group from week 3 (p<0.05). Transmission electron microscopy showed tighter gaps between vascular endothelial cells with swollen mitochondria in the OVX group from week 3. The MVD values of the OVX group decreased significantly compared with those of the control group only at week 12 (p=0.023). A weak positive correlation of Emaxand a strong positive correlation of Ktranswith MVD were found.ConclusionsCompared with semi-quantitative DCE-MRI, the quantitative DCE-MRI parameter Ktransis a more sensitive and accurate index for detecting early reduced perfusion in osteoporotic bone.
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影响因子：
4.8
作者：
Wáng YX;Griffith JF;Deng M;Yeung DK;Yuan J
通讯作者：
Yuan J
影响因子：
5.4
作者：
Barnes SL;Whisenant JG;Loveless ME;Yankeelov TE
通讯作者：
Yankeelov TE
影响因子：
5.9
作者：
Damilakis, John;Maris, Thomas G.;Karantanas, Apostolos H.
通讯作者：
Karantanas, Apostolos H.
影响因子：
6.2
作者：
Griffith, James F.;Yeung, David K. W.;Leung, Ping C.
通讯作者：
Leung, Ping C.
影响因子：
1.3
作者：
Liu, Yifei;Cao, Liji;Baeuerle, Tobias
通讯作者：
Baeuerle, Tobias