Identification and profiling of miRNAs during herbivory reveals jasmonate-dependent and -independent patterns of accumulation in Nicotiana attenuata.
Identification and profiling of miRNAs during herbivory reveals jasmonate-dependent and -independent patterns of accumulation in Nicotiana attenuata.
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DOI：
10.1186/1471-2229-12-209
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发表时间：
2012-11-07
影响因子：
5.3
通讯作者：
Kim SG
中科院分区：
文献类型：
--
作者：
Bozorov TA;Baldwin IT;Kim SG
植物microRNAs （miRNAs）在对环境胁迫的转录反应中起着关键作用。然而，mirna在昆虫食草性反应中的作用尚未得到充分探讨。为了鉴定草食反应性mirna，我们在生态模式植物烟叶（Nicotiana attenuata）中鉴定了保守的mirna，该植物与草食动物的相互作用在实验室和野外研究中都得到了很好的表征。我们鉴定了来自36个家族的59个mirna，以及两个内源性的mirna靶向的反式作用小干扰rna （tasiRNA）。我们通过定量PCR分析鉴定了来自专业食草动物Manduca sexta的前体和成熟miRNA对模拟攻击的反应，并使用缺乏茉莉酸生物合成的ir-aoc RNAi转化子来鉴定茉莉酸依赖性和非依赖性miRNA调控。表达分析表明，这些mirna和tasirna分别受到机械损伤和口腔分泌物的特异性调控，这些小rna以茉莉酸依赖或独立的方式积累。此外，cDNA微阵列分析表明，相应靶基因的表达模式与mirna和tasirna的积累相关。我们发现一组mirna和tasirna协调了靶基因的表达，这些基因参与了N. attenuata对食草动物攻击的反应。
Plant microRNAs (miRNAs) play key roles in the transcriptional responses to environmental stresses. However, the role of miRNAs in responses to insect herbivory has not been thoroughly explored. To identify herbivory-responsive miRNAs, we identified conserved miRNAs in the ecological model plant Nicotiana attenuata whose interactions with herbivores have been well-characterized in both laboratory and field studies. We identified 59 miRNAs from 36 families, and two endogenous trans-acting small interfering RNAs (tasiRNA) targeted by miRNAs. We characterized the response of the precursor and mature miRNAs to simulated attack from the specialist herbivore Manduca sexta by quantitative PCR analysis and used ir-aoc RNAi transformants, deficient in jasmonate biosynthesis, to identify jasmonate-dependent and -independent miRNA regulation. Expression analysis revealed that groups of miRNAs and tasiRNAs were specifically regulated by either mechanical wounding or wounding plus oral secretions from M. sexta larvae, and these small RNAs were accumulated in jasmonate-dependent or -independent manners. Moreover, cDNA microarray analysis indicated that the expression patterns of the corresponding target genes were correlated with the accumulation of miRNAs and tasiRNAs. We show that a group of miRNAs and tasiRNAs orchestrates the expression of target genes involved in N. attenuata’s responses to herbivore attack.
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DOI：
10.1073/pnas.1200363109
发表时间：
2012-06-12
影响因子：
11.1
作者：
Kallenbach, Mario;Bonaventure, Gustavo;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.
影响因子：
3.7
作者：
Kim SH;Lu HF;Alano CC
通讯作者：
Alano CC
影响因子：
11.6
作者：
Kang, Jin-Ho;Wang, Lei;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.
miRNA 和 siRNA 在植物生物和非生物胁迫反应中的作用。
DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
4.7
作者：
Khraiwesh, Basel;Zhu, Jian-Kang;Zhu, Jianhua
通讯作者：
Zhu, Jianhua
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP