Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Nile tilapia (Oreochromis niloticus)
Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Nile tilapia (Oreochromis niloticus)
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尼罗罗非鱼 (Oreochromis niloticus) 性连锁 DNA 标记的筛选和表征以及标记辅助选择
DOI：
10.1016/j.aquaculture.2014.05.035
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发表时间：
2014-09
期刊：
影响因子：
4.5
通讯作者：
Wang DS
中科院分区：
文献类型：
--
作者：
Yang C;Yang SJ;Li MH;Wang DS
尼罗罗非鱼是一种人工养殖的硬骨鱼，雄性比雌性长得更快、更大，单性养殖可以避免生长过程中的不必要繁殖。罗非鱼性别控制是水产养殖中的一个重要问题。在本研究中，随机扩增多态性DNA（RAPD）和扩增片段长度多态性（AFLP）指纹技术被用来筛选合并和个别的DNA样品从XX，XY，YY鱼的性连锁标记。通过序列分析和荧光原位杂交（FISH）技术，获得了4个与性连锁的标记（RAPD标记1和AFLP标记2、3、4），并定位在小染色体LG 23上。此外，根据LG 23上的支架7、29和101的序列设计了总共32对引物，并使用这些引物通过PCR筛选X和Y之间的遗传差异。其中之一（标记-5）在XX、XY和YY个体之间产生了可检测的差异。根据该序列特征扩增区（SCAR）设计了8对引物，成功转化为SCAR标记，并对已知基因型的不同杂交后代进行验证。随后，用标记-4和-5对来自中国不同养殖场的8个尼罗罗非鱼群体进行性别鉴定，结果一致率为76%~ 100%。根据XX（XX）× XY（XY）后代的重组率，估计标记-1、-2、-3、-4和-5距离性别决定位点（SD）分别约为5、3、5、2和0 cM。标记-5，其映射接近先前报道的SD（Eshel et al.，2012），用于遗传雄性罗非鱼（GMT）的选择性育种。
The Nile tilapia is a cultured teleost fish in which males grow faster and larger than females, and mono-sex culturing could avoid unwanted reproduction during grow-out. Sex control in tilapia is an important issue in aquaculture. In the present study, randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) fingerprinting were used to screen pooled and individual DNA samples from XX, XY, and YY fish for sex-linked markers. Four sex-linked markers (Marker-1 from RAPD and Markers-2, -3, and -4 from AFLP) were obtained and mapped to LG23, a small chromosome, by sequence analysis and fluorescence in situ hybridization (FISH). In addition, a total of 32 pairs of primers were designed based on the sequences of scaffolds 7, 29, and 101 on LG23, and these were used to screen genetic differences between X and Y by PCR. One of these (Marker-5) produced a detectable difference between XX, XY, and YY individuals. Eight pairs of primers based on the sequence characterized amplified region (SCAR) were designed and successfully converted to SCAR markers, which were used to sex progeny from different crosses of known genotypes for validation. Subsequently, Markers-4 and -5 were used to sex eight populations of Nile tilapia collected from different fish farms in China, which gave concordance rates that ranged from 76 to 100%. Based on the recombination rate derived from the progeny of XX (♀) × XY (♂), Markers-1, -2, -3, -4, and -5 were estimated to be around 5, 3, 5, 2 and 0 cM away from the sex-determining locus (SD), respectively. Marker-5, which mapped close to the SD reported previously (Eshel et al., 2012), was used for selective breeding of genetic male tilapia (GMT).
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尼罗罗非鱼（Oreochromis niloticus）中性别连接的AFLP标记的遗传和物理图。
影响因子：
3
作者：
Lee, Bo-Young;Coutanceau, Jean-Pierre;Ozouf-Costaz, Catherine;D'Cotta, Helena;Baroiller, Jean-Francois;Kocher, Thomas D.
通讯作者：
Kocher, Thomas D.
影响因子：
4.5
作者：
Beardmore, JA;Mair, GC;Lewis, RI
通讯作者：
Lewis, RI
影响因子：
2.6
作者：
H. Zhu;D. Ma;J. Gui
通讯作者：
H. Zhu;D. Ma;J. Gui
影响因子：
4.4
作者：
Liu F;Sun F;Li J;Xia JH;Lin G;Tu RJ;Yue GH
通讯作者：
Yue GH
YY超雄性和XY全雄性黄颡鱼（Pelteobagrus fulvidraco (Richardson)）规模化养殖的性别比遗传调控
影响因子：
3
作者：
Liu, Hanqin;Guan, Bo;Chen, Hongxi
通讯作者：
Chen, Hongxi