A receptor protein tyrosine kinase implicated in the segmental patterning of the hindbrain and mesoderm.
A receptor protein tyrosine kinase implicated in the segmental patterning of the hindbrain and mesoderm.
复制标题
一种受体蛋白酪氨酸激酶，与后脑和中胚层的节段模式有关。
作者：
M. Nieto;P. Gilardi;P. Charnay;D. Wilkinson
脊椎动物的后脑和轴旁中胚层的模式形成是通过一系列重复片段的形成而发生的。这些分割过程在形态学水平上似乎不同，因为后脑节，菱形，形成的神经上皮细胞细分成车厢，而中胚层体节形成的间充质细胞成上皮球的顺序聚集。先前的研究表明编码转录因子的基因参与了后脑节段的发育，但对参与体节形成的基因却一无所知。细胞相互作用和信号转导必须是后脑分割的一个重要方面，所以我们已经筛选了在发育中的小鼠胚胎中以菱形限制模式表达的酪氨酸激酶。我们已经确定了一种受体蛋白酪氨酸激酶，Sek，在菱体3和5中具有较高的相对表达水平。这种交替模式是建立巧合，在空间和时间上，与Krox-20的表达，锌指基因的表达之前的形态形成的菱形。此外，Sek表达发生在几个其他发育组织中，包括在发育中的前脑、脊髓、早期中胚层和前体前中胚层（节段板）中的动态调节。后一种表达出现在两条条纹中，这两条条纹与体节的形成相关，并预示着体节的形成。Sek的表达最初发生在整个假定的体节，然后变得受限于前部，最后随着最终体节的形成而下调。这些数据表明，尽管形态学差异的分割后脑和中胚层，Sek是参与这两个组织的节段图案。
Pattern formation in the hindbrain and paraxial mesoderm of vertebrates occurs by the formation of a series of repeated segments. These processes of segmentation appear different at the morphological level, since hindbrain segments, the rhombomeres, form by the subdivision of the neural epithelium into compartments, whereas the mesodermal somites form by the sequential aggregation of mesenchymal cells into epithelial balls. Previous studies have implicated genes encoding transcription factors in the development of hindbrain segments, but nothing is known of genes involved in the formation of somites. Cellular interactions and signal transduction must be an important aspect of hindbrain segmentation, so we have screened for tyrosine kinases expressed in rhombomere-restricted patterns in the developing mouse embryo. We have identified a receptor protein tyrosine kinase, Sek, that has high relative levels of expression in rhombomeres 3 and 5. This alternating pattern is established coincidentally, both spatially and temporally, with the expression of Krox-20, a zinc-finger gene expressed prior to the morphological formation of rhombomeres. In addition, Sek expression occurs in several other developing tissues, including a dynamic regulation in the developing forebrain, spinal cord, early mesoderm and anterior presomitic mesoderm (segmental plate). The latter expression occurs in two stripes that correlate with, and presage, the formation of somites. Sek expression initially occurs throughout the presumptive somite, then becomes restricted anteriorly, and finally is down-regulated as the definitive somite is formed. These data suggest that despite the morphological differences in the segmentation of the hindbrain and mesoderm, Sek is involved in the segmental patterning of both of these tissues.
登录
查看更多内容
鸡胚激酶 5 的鉴定，一种 Eph 家族发育调节受体型酪氨酸激酶。
DOI：
10.1091/mbc.2.7.523
发表时间：
1991
期刊：
Cell regulation
影响因子：
--
作者：
Pasquale,EB
通讯作者：
Pasquale,EB
影响因子：
10.5
作者：
NOCKA, K;MAJUMDER, S;BESMER, P
通讯作者：
BESMER, P
J1/肌腱蛋白相关分子不负责鸡胚胎中神经嵴细胞或运动轴突的分段模式。
DOI：
10.1242/dev.107.2.309
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Stern,CD;Norris,WE;Bronner-Fraser,M;Carlson,GJ;Faissner,A;Keynes,RJ;Schachner,M
通讯作者：
Schachner,M
影响因子：
4.6
作者：
H. Nornes;G. Dressler;E. Knapik;U. Deutsch;Peter Gruss
通讯作者：
H. Nornes;G. Dressler;E. Knapik;U. Deutsch;Peter Gruss
T-钙粘蛋白的表达与鸟类胚胎躯干中神经嵴细胞的迁移交替出现。
DOI：
10.1242/dev.111.1.15
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ranscht,B;Bronner-Fraser,M
通讯作者：
Bronner-Fraser,M