Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution.
Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution.
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DOI：
10.1007/s00253-012-4502-5
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发表时间：
2013-03
影响因子：
5
通讯作者：
Brynjolfsson, Sigurour
中科院分区：
文献类型：
--
作者：
Fu, Weiqi;Guomundsson, Olafur;Paglia, Giuseppe;Herjolfsson, Gisli;Andresson, Olafur S.;Palsson, Bernhard O.;Brynjolfsson, Sigurour
关键词：
对于从高等植物和藻类中获得类胡萝卜素如β-胡萝卜素和叶黄素用于全球市场存在特别高的兴趣。众所周知，β-胡萝卜素可以在响应于应激光条件的绿色微藻盐藻中过量产生。然而，关于光质量对类胡萝卜素代谢的影响知之甚少，例如，窄光谱红光在这项研究中，我们提出了UPLC-UV-MS数据从D。salina与提出的绿色微生物莱茵衣藻类胡萝卜素代谢途径一致。研究了红光发光二极管（LED）照明对藻生长速率和生物量的影响，确定了最佳的光通量。盐沼生长我们发现，主要的类胡萝卜素的变化平行于叶绿素B含量和红光光子应力单独在高水平是不能够上调类胡萝卜素的积累，大概是由于严重的光损伤。我们已经发现，将红色LED（75%）与蓝色LED（25%）组合允许以更高的总光子通量生长。额外的蓝光而不是红光导致β-胡萝卜素和叶黄素积累增加，长期迭代胁迫（适应性实验室进化）的应用产生了D.盐藻在蓝光和红光组合下类胡萝卜素积累增加。本文的在线版本（doi：10.1007/s 00253 -012-4502-5）包含补充材料，可供授权用户使用。
There is a particularly high interest to derive carotenoids such as β-carotene and lutein from higher plants and algae for the global market. It is well known that β-carotene can be overproduced in the green microalga Dunaliella salina in response to stressful light conditions. However, little is known about the effects of light quality on carotenoid metabolism, e.g., narrow spectrum red light. In this study, we present UPLC-UV-MS data from D. salina consistent with the pathway proposed for carotenoid metabolism in the green microalga Chlamydomonas reinhardtii. We have studied the effect of red light-emitting diode (LED) lighting on growth rate and biomass yield and identified the optimal photon flux for D. salina growth. We found that the major carotenoids changed in parallel to the chlorophyll b content and that red light photon stress alone at high level was not capable of upregulating carotenoid accumulation presumably due to serious photodamage. We have found that combining red LED (75 %) with blue LED (25 %) allowed growth at a higher total photon flux. Additional blue light instead of red light led to increased β-carotene and lutein accumulation, and the application of long-term iterative stress (adaptive laboratory evolution) yielded strains of D. salina with increased accumulation of carotenoids under combined blue and red light. The online version of this article (doi:10.1007/s00253-012-4502-5) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
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影响因子：
5
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
Craford, M. George
影响因子：
3.4
作者：
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通讯作者：
Katoh, S
影响因子：
56.9
作者：
Merchant, Sabeeha S.;Prochnik, Simon E.;Grossman, Arthur R.
通讯作者：
Grossman, Arthur R.