Molecular characterization of a novel Muscovy duck parvovirus isolate: evidence of recombination between classical MDPV and goose parvovirus strains.
Molecular characterization of a novel Muscovy duck parvovirus isolate: evidence of recombination between classical MDPV and goose parvovirus strains.
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番鸭细小病毒新型分离株的分子特征：经典 MDPV 与鹅细小病毒株之间重组的证据
DOI：
10.1186/s12917-017-1238-6
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发表时间：
2017-11-09
影响因子：
2.6
通讯作者：
Zhu G
中科院分区：
文献类型：
--
作者：
Wang J;Ling J;Wang Z;Huang Y;Zhu J;Zhu G
番鸭细小病毒(MDPV)和鹅细小病毒(GPV)分别是番鸭细小病毒病和德兹氏病的重要病原，对水禽养殖业造成巨大的经济损失。与GPV相比，MDPV分离株的完整基因组序列数据仍然有限，它们的系统发育关系在很大程度上仍不清楚。本研究对2006年从中国雏番鸭身上分离到的致病番鸭新城疫病毒ZW株的全基因组进行了克隆、测序，并与其他经典的新分离株和GPV株进行了比较。ZW株基因组全长5071个核苷酸，比致病株YY株(5075个核苷酸)短。ZW菌株产生的4个缺失核苷酸均位于ITR回文茎的碱基配对位置，不影响发夹结构的形成。重组分析表明，ZW株来源于经典的MDPV株和GPV株的基因重组。MDPV YY株为主要亲本，强毒株YZ99-6、B株和疫苗株SYG61v不同程度地起次要亲本作用。在菌株ZW中检测到两个重组位点，小的重组位点位于P9启动子周围，大的重组位点位于VP3基因的中间。SYG61V株是一种用于预防鹅细小病毒病的疫苗株。该菌株被发现只参与在P9启动子区域检测到的重组事件。对ZW株与其他MDPV和GPV经典毒株进行了系统发育分析。这一结果支持了In Silicon复合分析的结论。MDPV ZW株是一种新的重组细小病毒，其大部分基因组来源于经典的MDPV株。两株GPV强毒株和一株疫苗株不同程度地参与了重组过程。
Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are important etiological agents for Muscovy duck parvoviral disease and Derzsy’s disease, respectively; both of which can cause substantial economic losses in waterfowl industry. In contrast to GPV, the complete genomic sequence data of MDPV isolates are still limited and their phylogenetic relationships largely remain unknown. In this study, the entire genome of a pathogenic MDPV strain ZW, which was isolated from a deceased Muscovy duckling in 2006 in China, was cloned, sequenced, and compared with that of other classical MDPV and GPV strains. The genome of strain ZW comprises of 5071 nucleotides; this genome was shorter than that of the pathogenic MDPV strain YY (5075 nt). All the four deleted nucleotides produced in strain ZW are located at the base-pairing positions in the palindromic stem of inverted terminal repeats (ITR) without influencing the formation of a hairpin structure. Recombination analysis revealed that strain ZW originated from genetic recombination between the classical MDPV and GPV strain. The YY strain of MDPV acts as the major parent, whereas the virulent strains YZ99–6 and B and the vaccine strain SYG61v of GPV act as the minor parents in varying degrees. Two recombination sites were detected in strain ZW, with the small recombination site surrounding the P9 promoter, and the large recombination site situated in the middle of the VP3 gene. The SYG61V strain is a vaccine strain used for preventing goose parvoviral disease. This strain was found to be solely involved in the recombination event detected in the P9 promoter region. Phylogenetic analyses between strain ZW and other classical strains of MDPV and GPV were performed. The results supported the in silico recombination analysis conclusion. MDPV Strain ZW is a novel recombinant parvovirus, and the bulk of its genome originates from the classical MDPV strain. Two virulent strains and a vaccine strain of GPV were involved in the recombination process in varying degrees.
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影响因子：
5.4
作者：
Li, Long;Qiu, Jianming;Pintel, David J.
通讯作者：
Pintel, David J.
影响因子：
2.8
作者：
Moreno, Ana;Franzo, G.;Cecchinato, M.
通讯作者：
Cecchinato, M.
影响因子：
5.4
作者：
Qiu, JM;Cheng, F;Pintel, D
通讯作者：
Pintel, D
影响因子：
5.3
作者：
Martin DP;Murrell B;Golden M;Khoosal A;Muhire B
通讯作者：
Muhire B
影响因子：
3.3
作者：
Quinteros JA;Lee SW;Markham PF;Noormohammadi AH;Hartley CA;Legione AR;Coppo MJC;Vaz PK;Browning GF
通讯作者：
Browning GF