A panel of 16 full-length HCV genomes was characterized in China belonging to genotypes 1-6 including subtype 2f and two novel genotype 6 variants.
A panel of 16 full-length HCV genomes was characterized in China belonging to genotypes 1-6 including subtype 2f and two novel genotype 6 variants.
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DOI：
10.1016/j.meegid.2013.08.014
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发表时间：
2013-12
影响因子：
3.2
通讯作者：
Lu, Ling
中科院分区：
文献类型：
--
作者：
Xu, Ru;Tong, Wangxia;Gu, Lin;Li, Chunhua;Fu, Yongshui;Lu, Ling
在这项研究中，我们用重叠聚合酶链式反应和DNA测序分析了16株丙型肝炎病毒分离株的全长基因组。获得的基因组长度为9,414-9,628个核苷酸，每个基因组包含一个9,021-9,102个核苷酸的ORF。9个基因组代表常见的亚型1a、1b、2a、2b、3a、3b和6a，而7个基因组代表不常见的谱系1c、2f、4d和5a，以及两个新的基因6变异体。GZ51969和GZ52540为1b亚型分离株，分属A和B两个独特的聚类群，分别占中国1b感染病例的29.5%和59.5%。ZS542和GZ98799代表了临时指定的2f亚型的前两个完整基因组。ZS96和ZS202是新的6型变异体，可能符合两个新的亚型。ZS17、ZS537和ZS631代表3个外来亚型，即1c、4d和5a，这三个亚型是本研究中首次在中国中发现的，可能是最近因全球化而引入的。综上所述，这些结果通过对中国丙型肝炎病毒全长基因组的测序证实了丙型肝炎病毒在中国中的分类谱系的多样性。这些谱系代表6个基因类型、11个亚型和两个新的变种。它们的特点是为了更好地了解丙型肝炎病毒的遗传变异模式，以及未来可能的研究应用。
In this study, we characterized the full-length genomes of 16 HCV isolates obtained from patients in a single hospital in China using overlapping PCR followed by DNA sequencing. The obtained genomes are 9,414-9,628 nucleotides in length, and each genome contains a single ORF of 9,021-9,102 nucleotides. Nine genomes represent the common subtypes 1a, 1b, 2a, 2b, 3a, 3b, and 6a, while seven represent the infrequent lineages 1c, 2f, 4d, and 5a, and two novel genotype 6 variants. GZ51969 and GZ52540 are subtype 1b isolates belonging to two unique clusters designated A and B, which account for 29.5% and 59.5% of the 1b infections in China, respectively. ZS542 and GZ98799 represent the first two complete genomes of the provisionally assigned subtype 2f. ZS96 and ZS202 are novel genotype 6 variants that may qualify for two new subtypes. ZS17, ZS537, and ZS631 represent three alien subtypes, namely, 1c, 4d, and 5a, which were detected in China for the first time in this study and may have been recently introduced as a result of globalization. Taken together, these results confirmed a large variety of HCV taxonomic lineages in China through the sequencing of their full-length genomes. These lineages represent six genotypes, 11 subtypes, and two novel variants. They were characterized for achieving a better understanding of the HCV genetic variation patterns and for possible future research applications.
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影响因子：
12.7
作者：
Xia, Xueshan;Lu, Ling;Tee, Kok Keng;Zhao, Wenhua;Wu, Jianguo;Yu, Jing;Li, Xiaojie;Lin, Yixiong;Mukhtar, Muhammad Mahmood;Hagedorn, Curt H.;Takebe, Yutaka
通讯作者：
Takebe, Yutaka
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
DOI：
10.1099/vir.0.047506-0
发表时间：
2013-01
期刊：
The Journal of general virology
影响因子：
--
作者：
Wang H;Yuan Z;Barnes E;Yuan M;Li C;Fu Y;Xia X;Li G;Newton PN;Vongsouvath M;Klenerman P;Pybus OG;Murphy D;Abe K;Lu L
通讯作者：
Lu L
影响因子：
3.8
作者：
Lu, L;Nakano, T;Hagedorn, CH
通讯作者：
Hagedorn, CH
影响因子：
12.7
作者：
Xia, Xueshan;Zhao, Wenhua;Lu, Ling
通讯作者：
Lu, Ling