The unique HCV genotype distribution and the discovery of a novel subtype 6u among IDUs co-infected with HIV-1 in Yunnan, China.
The unique HCV genotype distribution and the discovery of a novel subtype 6u among IDUs co-infected with HIV-1 in Yunnan, China.
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DOI：
10.1002/jmv.21204
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发表时间：
2008-07
影响因子：
12.7
通讯作者：
Takebe, Yutaka
中科院分区：
文献类型：
--
作者：
Xia, Xueshan;Lu, Ling;Tee, Kok Keng;Zhao, Wenhua;Wu, Jianguo;Yu, Jing;Li, Xiaojie;Lin, Yixiong;Mukhtar, Muhammad Mahmood;Hagedorn, Curt H.;Takebe, Yutaka
云南省是中国HIV-1流行病的中心，也是向世界其他地区贩运毒品的中心。在全省6个地（市）共抽取132名静脉吸毒者进行HCV和HIV-1血清学检测，阳性率分别为93.94%和68.18%（P <0.001）。对89例注射吸毒者进行了HCV和HIV-1共感染的检测，并对其中的几个HCV片段进行了扩增和测序。对82例静脉吸毒者进行了HCV 5′ NCR-C和NS 5 B区基因序列测定。系统发育分析显示80名注射吸毒者的基因型一致。其中1a、1b、3a、3b、6a、6 n基因型分别为1例（1.25%）、16例（20%）、19例（23.75%）、24例（30%）、4例（5%）、9例（11.25%）和7例（8.75%）。在两个注射吸毒者中，基因分型结果不一致，提示混合HCV感染或重组。从北到南，从东到西，HCV 1b阳性率呈下降趋势。基因3型和基因6型菌株在南部地区较为常见。新的6 u亚型毒株仅在与缅甸接壤的德宏州检出。我们的研究结果显示了一个独特的模式，HCV基因型分布，这是类似于在东南亚国家，但不同于在中国的一般人群。贩毒途径和由此导致的HIV-1感染的高流行率可能促成了HCV基因型分布的这种模式。
The Yunnan province is the epicenter of HIV-1 epidemics in China and a center for drug trafficking to the other parts of the world. In six prefectures of this province, a total of 132 IDUs were recruited to determine the seroprevalence of HCV and HIV-1 and the positive rates were 93.94% and 68.18%, respectively (P <0.001). Co-infection with HCV and HIV-1 was found among 89 IDUs, of whom several HCV fragments were amplified and sequenced. Sequences of the HCV 5′NCR-C and NS5B region were determined from 82 IDUs. Phylogenetic analyses showed consistent genotyping among 80 IDUs. Among them HCV genotypes 1a, 1b, 3a, 3b, 6a, 6n, and a tentatively assigned novel 6u subtype were found in 1 (1.25%), 16 (20%), 19 (23.75%), 24 (30%), 4 (5%), 9 (11.25%) and 7 (8.75%) individuals, respectively. In two IDUs, genotyping results were discordant, suggesting mixed HCV infections or recombination. The proportion of patients with HCV 1b tended to decrease from the north to south and from the east to west in this province. Genotype 3 and 6 strains were more frequent in the southern prefectures. The novel subtype 6u strains were only detected in Dehong which borders Myanmar. Our findings showed a unique pattern of HCV genotype distribution, which is similar to that in the southeastern Asian countries but distinct from that among the general population in China. Routes of drug trafficking and the resulting high prevalence of HIV-1 infection may have contributed to this pattern of HCV genotype distribution.
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影响因子：
28.2
作者：
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通讯作者：
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影响因子：
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