Flipping the switch: tools for detecting small molecule inhibitors of staphylococcal virulence.
Flipping the switch: tools for detecting small molecule inhibitors of staphylococcal virulence.
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DOI：
10.3389/fmicb.2014.00706
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发表时间：
2014
影响因子：
5.2
通讯作者：
Horswill AR
中科院分区：
文献类型：
--
作者：
Quave CL;Horswill AR
通过辅助基因调节因子群体感应级联的表达，金黄色葡萄球菌能够产生大量的酶、溶血素和免疫调节剂，这对于它在宿主组织中传播和致病是必不可少的。许多人主张发现和开发群体感应抑制剂(QSIS)，以增强现有的抗生素作为辅助治疗。在这里，我们讨论可用于进行筛查以识别此类QSIS的最先进的工具。例如荧光记者、自动诱导肽生产的MS检测、检测溶血素和脂肪酶的琼脂平板法、从上清液中检测溶血素的高效液相色谱检测，以及检测对人角质形成细胞的损伤(或其缓解)的细胞毒性检测。除了提供对这些不同方法的描述外，我们还讨论了它们对用于鉴定新的QSIS的低通量、中通量和高通量筛选努力的适应性。
Through the expression of the accessory gene regulator quorum sensing cascade, Staphylococcus aureus is able to produce an extensive array of enzymes, hemolysins and immunomodulators essential to its ability to spread through the host tissues and cause disease. Many have argued for the discovery and development of quorum sensing inhibitors (QSIs) to augment existing antibiotics as adjuvant therapies. Here, we discuss the state-of-the-art tools that can be used to conduct screens for the identification of such QSIs. Examples include fluorescent reporters, MS-detection of autoinducing peptide production, agar plate methods for detection of hemolysins and lipase, High performance liquid chromatography-detection of hemolysins from supernatants, and cell-toxicity assays for detecting damage (or relief thereof) against human keratinocyte cells. In addition to providing a description of these various approaches, we also discuss their amenability to low-, medium-, and high-throughput screening efforts for the identification of novel QSIs.
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影响因子：
11.3
作者：
Cheung GY;Joo HS;Chatterjee SS;Otto M
通讯作者：
Otto M
影响因子：
2.7
作者：
Cech NB;Junio HA;Ackermann LW;Kavanaugh JS;Horswill AR
通讯作者：
Horswill AR
影响因子：
5.4
作者：
Kjaerulff L;Nielsen A;Mansson M;Gram L;Larsen TO;Ingmer H;Gotfredsen CH
通讯作者：
Gotfredsen CH
影响因子：
7.3
作者：
Gordon, Christopher P.;Williams, Paul;Chan, Weng C.
通讯作者：
Chan, Weng C.
影响因子：
3.1
作者：
Lauderdale, Katherine J.;Boles, Blaise R.;Horswill, Alexander R.
通讯作者：
Horswill, Alexander R.