Western and heart healthy dietary patterns differentially affect the expression of genes associated with lipid metabolism, interferon signaling and inflammation in the jejunum of Ossabaw pigs.
Western and heart healthy dietary patterns differentially affect the expression of genes associated with lipid metabolism, interferon signaling and inflammation in the jejunum of Ossabaw pigs.
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DOI：
10.1016/j.jnutbio.2020.108577
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Lichtenstein AH
中科院分区：
文献类型：
--
作者：
Ye S;Matthan NR;Lamon-Fava S;Aguilar GS;Turner JR;Walker ME;Chai Z;Lakshman S;Urban JF Jr;Lichtenstein AH
饮食质量和他汀类药物治疗是冠状动脉疾病（CAD）进展的公认调节剂，但其对胃肠道的影响以及可能影响CAD进展的后续后遗症相对未被探索。为了解决这一差距，Ossabaw猪（N = 32）被随机分配接受等热量的西式饮食（WD;高饱和脂肪，精制碳水化合物和胆固醇，低纤维）或心脏健康型饮食（HHD;高不饱和脂肪，全谷物，水果和蔬菜，补充鱼油，低胆固醇），有或没有阿托伐他汀，持续6个月。在研究结束时，在NextSeq 500平台上用100个碱基对单端读数在分离的猪空肠粘膜中进行RNA测序。两因素edgeR分析显示，饮食模式导致三个差异表达的基因与脂质代谢（SCD，FADS 1，和SQLE）。这些基因的表达与心脏代谢危险因素和动脉粥样硬化病变的严重程度有关。随后的基因富集分析表明，与HHD相比，WD导致更高的干扰素信号传导和炎症，其中一些基因与血清TNF-α和/或hsCRP浓度显著相关，但与动脉粥样硬化病变严重程度无关。阿托伐他汀治疗对基因表达没有显著影响，也没有发现它与饮食模式的相互作用。总之，西方和心脏健康型的饮食模式差异影响的基因表达与脂质代谢，干扰素信号，和炎症的Ossabaw猪空肠。
Diet quality and statin therapy are established modulators of coronary artery disease (CAD) progression, but their effect on the gastrointestinal tract and subsequent sequelae that could affect CAD progression are relatively unexplored. To address this gap, Ossabaw pigs (N = 32) were randomly assigned to receive isocaloric amounts of a Western-type diet (WD; high in saturated fat, refined carbohydrate, and cholesterol, and low in fiber) or a heart healthy-type diet (HHD; high in unsaturated fat, whole grains, fruits and vegetables, supplemented with fish oil, and low in cholesterol), with or without atorvastatin, for 6 months. At the end of the study, RNA sequencing with 100 base pair single end reads on NextSeq 500 platform was conducted in isolated pig jejunal mucosa. A two-factor edgeR analysis revealed that the dietary patterns resulted in three differentially expressed genes related to lipid metabolism (SCD, FADS1, and SQLE). The expression of these genes was associated with cardiometabolic risk factors and atherosclerotic lesion severity. Subsequent gene enrichment analysis indicated the WD, compared to the HHD, resulted in higher interferon signaling and inflammation, with some of these genes being significantly associated with serum TNF-α and/or hsCRP concentrations, but not atherosclerotic lesion severity. No significant effect of atorvastatin therapy on gene expression, nor its interaction with dietary patterns, was identified. In conclusion, Western and heart healthy-type dietary patterns differentially affect the expression of genes associated with lipid metabolism, interferon signaling, and inflammation in the jejunum of Ossabaw pigs.
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