Temporal patterns of lipoperoxidation and antioxidant enzymes are modified in the hippocampus of vitamin A-deficient rats.
Temporal patterns of lipoperoxidation and antioxidant enzymes are modified in the hippocampus of vitamin A-deficient rats.
复制标题
DOI：
10.1002/hipo.20571
复制
发表时间：
2009-09
期刊：
影响因子：
3.5
通讯作者：
Anzulovich, Ana C.
中科院分区：
文献类型：
--
作者：
Navigatore Fonzo, Lorena S.;Golini, Rebeca S.;Delgado, Silvia M.;Ponce, Ivana T.;Bonomi, Myrtha R.;Rezza, Irma G.;Gimenez, Maria S.;Anzulovich, Ana C.
动物可以通过记忆和学习过程以及内源性计时机制使自己的行为适应环境中可预测的时间波动。海马在记忆和学习中发挥着关键作用，特别容易受到氧化应激的影响。作为补偿，在该大脑区域检测到抗氧化酶活性，例如过氧化氢酶 (CAT) 和谷胱甘肽过氧化物酶 (GPx)。大脑中抗氧化酶活性以及谷胱甘肽和脂质过氧化物水平的每日节律已被描述。在这里，我们研究了大鼠海马中脂过氧化、CAT 和 GPx 表达和活性的昼夜变化，以及内源性时钟的两个关键组成部分 BMAL1 和 PER1 的时间波动，并评估维生素 A 缺乏可能在多大程度上影响其幅度或相位。在 24 小时内处死对照组、维生素 A 缺乏组和补充维生素 A 组的 Holtzman 雄性大鼠。每 4 或 5 小时测定大鼠海马中时钟蛋白、脂过氧化、CAT 和 GPx mRNA、蛋白和活性的每日水平。还对三组大鼠海马中 RARα 和 RXRβ 的基因表达进行了定量。我们的结果显示 BMAL1 和 PER1 蛋白表达的显着每日变化。还在大鼠海马中观察到节律性脂质过氧化、CAT 和 GPx 的表达和活性。维生素 A 缺乏降低了 RXRβ mRNA 水平以及 BMAL1 和 PER1 每日振荡幅度，使脂质过氧化的每日峰值发生相移，并对振荡 CAT 和 GPx mRNA、蛋白质和活性产生不同的影响。了解维生素 A 缺乏如何影响海马体昼夜节律基因表达可能会对神经生物学、营养学和时间生物学领域产生影响，首次强调了营养因素（例如膳食微量营养素）在调节大脑记忆和学习相关区域昼夜节律参数中的重要性。
Animals can adapt their behavior to predictable temporal fluctuations in the environment through both, memory-and-learning processes and an endogenous time-keeping mechanism. Hippocampus plays a key role in memory and learning and is especially susceptible to oxidative stress. In compensation, antioxidant enzymes activity, such as Catalase (CAT) and Glutathione peroxidase (GPx), has been detected in this brain region. Daily rhythms of antioxidant enzymes activitiy, as well as of glutathione and lipid peroxides levels, have been described in brain. Here, we investigate day/night variations in lipoperoxidation, CAT and GPx expression and activity, as well as the temporal fluctuations of two key components of the endogenous clock, BMAL1 and PER1, in the rat hippocampus and evaluate to which extent vitamin A deficiency may affect their amplitude or phase. Holtzman male rats from control, vitamin A-deficient and vitamin A-refed groups were sacrificed throughout a 24-h period. Daily levels of clock proteins, lipoperoxidation, CAT and GPx mRNA, protein, and activity, were determined in the rat hippocampus obtained every 4 or 5 h. Gene expression of RARα and RXRβ was also quantified in the hippocampus of the three groups of rats. Our results show significant daily variations of BMAL1 and PER1 protein expression. Rhythmic lipoperoxidation, CAT, and GPx, expression and activity, were also observed in the rat hippocampus. Vitamin A deficiency reduced RXRβ mRNA level, as well as the amplitude of BMAL1 and PER1 daily oscillation, phase-shifted the daily peak of lipoperoxidation, and had a differential effect on the oscillating CAT and GPx mRNA, protein, and activity. Learning how vitamin A deficiency affects the circadian gene expression in the hippocampus may have an impact on the neurobiology, nutritional and chronobiology fields, emphasizing for the first time the importance of nutritional factors, such as dietary micronutrients, in the regulation of circadian parameters in this brain memory-and-learning-related region.
登录
查看更多内容
DOI：
10.1016/s0091-6773(73)80164-6
发表时间：
1973-01-01
期刊：
BEHAVIORAL BIOLOGY
影响因子：
--
作者：
HOLLOWAY, FA;WANSLEY, RA
通讯作者：
WANSLEY, RA
影响因子：
3.3
作者：
Krezel, W;Kastner, P;Chambon, P
通讯作者：
Chambon, P
影响因子：
56.9
作者：
Balsalobre, A;Brown, SA;Schibler, U
通讯作者：
Schibler, U
影响因子：
64.5
作者：
HEYMAN, RA;MANGELSDORF, DJ;THALLER, C
通讯作者：
THALLER, C
DOI：
10.1073/pnas.93.17.9010
发表时间：
1996-08-20
影响因子：
11.1
作者：
Krezel, W;Dupe, V;Chambon, P
通讯作者：
Chambon, P