Migratory and adhesive properties of Xenopus laevis primordial germ cells in vitro.
Migratory and adhesive properties of Xenopus laevis primordial germ cells in vitro.
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DOI：
10.1242/bio.20135140
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发表时间：
2013-12-15
期刊：
影响因子：
2.4
通讯作者：
Pieler T
中科院分区：
文献类型：
--
作者：
Dzementsei A;Schneider D;Janshoff A;Pieler T
原始生殖细胞（PGCs）向性腺形成部位的定向迁移是研究细胞运动的一个有利的模型系统。PGCs的胚胎发育已经在不同的动物物种中进行了研究，包括小鼠、斑马鱼、非洲爪蟾和果蝇。在这项研究中，我们专注于物理性质的非洲爪蟾PGCs在其过渡过程中，从被动到主动迁移状态。从非洲爪蟾胚胎在发育阶段17-19的迁移前PGCs与阶段28-30的迁移PGCs进行比较，分离和表征的运动性和粘附性。使用单细胞力谱，我们观察到的PGC在达到迁移状态，如由减少附着到细胞外基质成分，如纤连蛋白，和减少粘附到体细胞内胚层细胞后的粘附性下降。从分离的PGCs的qPCR分析获得的数据显示，下调E-钙粘蛋白可能有助于这种细胞-细胞粘附的减弱。然而，有趣的是，使用体外迁移试验，我们发现X。乳牙PGC也可以独立于与其邻近细胞的特异性相互作用而发生。PGC发育过程中细胞粘附的减少伴随着细胞运动性的增强，这反映在水泡样突起的形成增加，并从电细胞基质阻抗传感（ECIS）以及延时图像分析中推断。细胞形状的时间变化，包括细胞体的收缩和扩张，揭示了体外迁移性PGC的更高程度的细胞动力学。
The directional migration of primordial germ cells (PGCs) to the site of gonad formation is an advantageous model system to study cell motility. The embryonic development of PGCs has been investigated in different animal species, including mice, zebrafish, Xenopus and Drosophila. In this study we focus on the physical properties of Xenopus laevis PGCs during their transition from the passive to the active migratory state. Pre-migratory PGCs from Xenopus laevis embryos at developmental stages 17–19 to be compared with migratory PGCs from stages 28–30 were isolated and characterized in respect to motility and adhesive properties. Using single-cell force spectroscopy, we observed a decline in adhesiveness of PGCs upon reaching the migratory state, as defined by decreased attachment to extracellular matrix components like fibronectin, and a reduced adhesion to somatic endodermal cells. Data obtained from qPCR analysis with isolated PGCs reveal that down-regulation of E-cadherin might contribute to this weakening of cell-cell adhesion. Interestingly, however, using an in vitro migration assay, we found that movement of X. laevis PGCs can also occur independently of specific interactions with their neighboring cells. The reduction of cellular adhesion during PGC development is accompanied by enhanced cellular motility, as reflected in increased formation of bleb-like protrusions and inferred from electric cell-substrate impedance sensing (ECIS) as well as time-lapse image analysis. Temporal alterations in cell shape, including contraction and expansion of the cellular body, reveal a higher degree of cellular dynamics for the migratory PGCs in vitro.
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影响因子：
3.4
作者：
Charras, Guillaume T.;Coughlin, Margaret;Mahadevan, L.
通讯作者：
Mahadevan, L.
影响因子：
1.6
作者：
HUTTER, JL;BECHHOEFER, J
通讯作者：
BECHHOEFER, J
DOI：
10.1073/pnas.1004401107
发表时间：
2010-09-14
影响因子：
11.1
作者：
Koebernick, Katja;Loeber, Jana;Pieler, Tomas
通讯作者：
Pieler, Tomas
影响因子：
0.7
作者：
Molyneaux, K;Wylie, C
通讯作者：
Wylie, C
影响因子：
14.8
作者：
Friedrichs, Jens;Helenius, Jonne;Muller, Daniel J.
通讯作者：
Muller, Daniel J.