Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation.
Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation.
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反向基因操作构建的高生长重配猪H3N2灭活疫苗的保护效果
DOI：
10.4142/jvs.2014.15.3.381
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发表时间：
2014
影响因子：
1.8
通讯作者：
Tong GZ
中科院分区：
文献类型：
--
作者：
Wen F;Ma JH;Yu H;Yang FR;Huang M;Zhou YJ;Li ZJ;Tong GZ
具有2009年H1N1大流行病毒基质基因的新型H3N2型猪流感病毒(SwIV)已在许多国家和美国多个州暴发期间分离到，这表明H3N2SwIV可能对公共卫生构成潜在威胁。由于南方中国是世界上最大的生猪生产国，应该开发有效的疫苗来防止猪感染H3N1亚型猪流感病毒，从而限制在农贸市场感染猪流感病毒的可能性。本研究采用基于质粒的反向遗传学方法获得了一株高生长重组病毒(GD/PR8)，并将其作为候选灭活疫苗进行了试验。用另一株H3N2SwIV毒株[A/Swin/黑龙江/1/05(H3N2)(HLJ/05)]攻击小鼠，评价该疫苗的免疫效果。用GD/PR8疫苗初免和加强免疫，均可产生高滴度的血凝抑制抗体和免疫球蛋白G抗体。观察到小鼠对H3N2SwIV完全保护，与模拟疫苗对照组相比，接种疫苗的小鼠的肺部病变和病毒载量显著减少。这些结果表明，gD/PR8疫苗有可能成为中国H3N2SwIV暴发的快速干预的候选疫苗。
Novel reassortant H3N2 swine influenza viruses (SwIV) with the matrix gene from the 2009 H1N1 pandemic virus have been isolated in many countries as well as during outbreaks in multiple states in the United States, indicating that H3N2 SwIV might be a potential threat to public health. Since southern China is the world's largest producer of pigs, efficient vaccines should be developed to prevent pigs from acquiring H3N2 subtype SwIV infections, and thus limit the possibility of SwIV infection at agricultural fairs. In this study, a high-growth reassortant virus (GD/PR8) was generated by plasmid-based reverse genetics and tested as a candidate inactivated vaccine. The protective efficacy of this vaccine was evaluated in mice by challenging them with another H3N2 SwIV isolate [A/Swine/Heilongjiang/1/05 (H3N2) (HLJ/05)]. Prime and booster inoculation with GD/PR8 vaccine yielded high-titer serum hemagglutination inhibiting antibodies and IgG antibodies. Complete protection of mice against H3N2 SwIV was observed, with significantly reduced lung lesion and viral loads in vaccine-inoculated mice relative to mock-vaccinated controls. These results suggest that the GD/PR8 vaccine may serve as a promising candidate for rapid intervention of H3N2 SwIV outbreaks in China.
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影响因子：
120.7
作者：
Thompson, WW;Shay, DK;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
6.7
作者：
Lakdawala SS;Lamirande EW;Suguitan AL Jr;Wang W;Santos CP;Vogel L;Matsuoka Y;Lindsley WG;Jin H;Subbarao K
通讯作者：
Subbarao K
影响因子：
3.7
作者：
Tian, GB;Zhang, SH;Chen, HL
通讯作者：
Chen, HL
影响因子：
6.4
作者：
Skowronski, Danuta M.;Janjua, Naveed Z.;Li, Yan
通讯作者：
Li, Yan
DOI：
10.1126/science.1176225
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garten RJ;Davis CT;Russell CA;Shu B;Lindstrom S;Balish A;Sessions WM;Xu X;Skepner E;Deyde V;Okomo-Adhiambo M;Gubareva L;Barnes J;Smith CB;Emery SL;Hillman MJ;Rivailler P;Smagala J;de Graaf M;Burke DF;Fouchier RA;Pappas C;Alpuche-Aranda CM;López-Gatell H;Olivera H;López I;Myers CA;Faix D;Blair PJ;Yu C;Keene KM;Dotson PD Jr;Boxrud D;Sambol AR;Abid SH;St George K;Bannerman T;Moore AL;Stringer DJ;Blevins P;Demmler-Harrison GJ;Ginsberg M;Kriner P;Waterman S;Smole S;Guevara HF;Belongia EA;Clark PA;Beatrice ST;Donis R;Katz J;Finelli L;Bridges CB;Shaw M;Jernigan DB;Uyeki TM;Smith DJ;Klimov AI;Cox NJ
通讯作者：
Cox NJ