Frequency dependence of CA3 spike phase response arising from h-current properties.
Frequency dependence of CA3 spike phase response arising from h-current properties.
复制标题
DOI：
10.3389/fncel.2013.00263
复制
发表时间：
2013
影响因子：
5.3
通讯作者：
Paulsen O
中科院分区：
文献类型：
--
作者：
Borel M;Guadagna S;Jang HJ;Kwag J;Paulsen O
海马神经元在θ振荡期间的放电相位编码空间信息。此外，在单个细胞中的突触输入的尖峰相位响应取决于超极化激活的混合阳离子电流（Ih），这是不同的CA3和CA1锥体神经元之间的表达。在这里，我们比较了这两种细胞类型的相位响应，以及它们的内在膜特性。我们发现，CA3和CA1锥体神经元都显示出响应负电流阶跃的电压暂降，但这种电压暂降在CA3细胞中显着较小。此外，与CA1锥体神经元相比，CA3锥体神经元具有不太突出的共振特性。这与两种细胞类型的Ih差异表达一致。尽管其独特的内在膜特性，CA3和CA1锥体神经元显示双向尖峰相位控制兴奋性电导输入在θ振荡。特别是，兴奋性输入提供的动态钳引起的膜电位振荡的下降阶段延迟了随后的尖峰近50毫拉德。该效应被证明是介导的Ih和被抵消的增加抑制电导驱动的膜电位振荡。使用我们的实验数据来喂养一个计算模型，我们表明，在CA 3和CA 1锥体神经元之间的Ih的差异可以预测这些细胞类型之间的相位响应特性的频率依赖性差异。我们实验证实了这种频率依赖性的尖峰相位控制在CA3神经元。因此，在新奇动物中观察到的theta频率的降低可能会将CA3尖峰相位响应从单向转换为双向，从而促进新上下文的编码。
The phase of firing of hippocampal neurons during theta oscillations encodes spatial information. Moreover, the spike phase response to synaptic inputs in individual cells depends on the expression of the hyperpolarization-activated mixed cation current (Ih), which differs between CA3 and CA1 pyramidal neurons. Here, we compared the phase response of these two cell types, as well as their intrinsic membrane properties. We found that both CA3 and CA1 pyramidal neurons show a voltage sag in response to negative current steps but that this voltage sag is significantly smaller in CA3 cells. Moreover, CA3 pyramidal neurons have less prominent resonance properties compared to CA1 pyramidal neurons. This is consistent with differential expression of Ih by the two cell types. Despite their distinct intrinsic membrane properties, both CA3 and CA1 pyramidal neurons displayed bidirectional spike phase control by excitatory conductance inputs during theta oscillations. In particular, excitatory inputs delivered at the descending phase of a dynamic clamp-induced membrane potential oscillation delayed the subsequent spike by nearly 50 mrad. The effect was shown to be mediated by Ih and was counteracted by increasing inhibitory conductance driving the membrane potential oscillation. Using our experimental data to feed a computational model, we showed that differences in Ih between CA3 and CA1 pyramidal neurons could predict frequency-dependent differences in phase response properties between these cell types. We confirmed experimentally such frequency-dependent spike phase control in CA3 neurons. Therefore, a decrease in theta frequency, which is observed in intact animals during novelty, might switch the CA3 spike phase response from unidirectional to bidirectional and thereby promote encoding of the new context.
登录
查看更多内容
影响因子：
16.2
作者：
Ekstrom, AD;Meltzer, J;Barnes, CA
通讯作者：
Barnes, CA
影响因子：
25
作者：
Kwag, Jeehyun;Paulsen, Ole
通讯作者：
Paulsen, Ole
影响因子：
3.7
作者：
Morellini, Fabio;Sivukhina, Elena;Schachner, Melitta
通讯作者：
Schachner, Melitta
影响因子：
3.8
作者：
Olvera-Cortés, E;Cervantes, M;González-Burgos, I
通讯作者：
González-Burgos, I
DOI：
10.1073/pnas.94.16.8918
发表时间：
1997-08-05
影响因子：
11.1
作者：
Mehta, MR;Barnes, CA;McNaughton, BL
通讯作者：
McNaughton, BL