Disruption of basal forebrain cholinergic neurons after traumatic brain injury does not compromise environmental enrichment-mediated cognitive benefits.
Disruption of basal forebrain cholinergic neurons after traumatic brain injury does not compromise environmental enrichment-mediated cognitive benefits.
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DOI：
10.1016/j.brainres.2020.147175
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发表时间：
2021-01-15
期刊：
影响因子：
2.9
通讯作者：
Bondi CO
中科院分区：
文献类型：
--
作者：
Moschonas EH;Leary JB;Memarzadeh K;Bou-Abboud CE;Folweiler KA;Monaco CM;Cheng JP;Kline AE;Bondi CO
与标准 (STD) 住房相比，环境富集 (EE) 可以减轻创伤性脑损伤 (TBI) 引起的内侧间隔 (MS) 胆碱乙酰转移酶 (ChAT) 细胞的损失，并增强空间学习和记忆。基底前脑胆碱能神经元 (BFCN) 是否是 TBI 后 EE 诱导益处的重要介质需要进一步研究。麻醉雌性大鼠被随机分配接受免疫毒素 192-IgG-皂草素（SAP；1.0 μL 中 0.22 μg）或载体（VEH；1.0 μL IgG）的间隔内输注，随后立即进行皮质冲击（4m/s 时变形深度为 2.8 mm）或假损伤，并分为 EE 和 STD 住房。术后第 14-19 天评估空间学习和记忆保留。 MS ChAT+ 细胞在 3 周时进行定量。 SAP 显着减少了 EE 组和 STD 组的 ChAT+ 细胞。与 STD 饲养组相比，无论输注 VEH 或 SAP，EE 组的认知表现均有所改善（p < 0.05）。 TBI + EE + SAP 和 TBI + EE + VEH 组之间（p > 0.05）或 TBI + STD + SAP 和 TBI + STD + VEH 组之间（p > 0.05）没有发现认知差异。这些数据表明，尽管 MS ChAT+ 细胞大量丢失，但 EE 介导的认知恢复益处并未受到损害。
Environmental enrichment (EE) attenuates traumatic brain injury (TBI)-induced loss of medial septal (MS) choline acetyltransferase (ChAT)-cells and enhances spatial learning and memory vs. standard (STD) housing. Whether basal forebrain cholinergic neurons (BFCNs) are important mediators of EE-induced benefits after TBI requires further investigation. Anesthetized female rats were randomly assigned to intraseptal infusions of the immunotoxin 192-IgG-saporin (SAP; 0.22 μg in 1.0 μL) or vehicle (VEH; 1.0 μL IgG) followed immediately by a cortical impact (2.8 mm deformation depth at 4m/s) or sham injury and divided into EE and STD housing. Spatial learning and memory retention were assessed on post-operative days 14–19. MS ChAT+ cells were quantified at 3 weeks. SAP significantly reduced ChAT+ cells in both the EE and STD groups. Cognitive performance was improved in the EE groups, regardless of VEH or SAP infusion, vs. the STD-housed groups (p’s < 0.05). No cognitive differences were revealed between the TBI + EE + SAP and TBI + EE + VEH groups (p > 0.05) or between the TBI + STD + SAP and TBI + STD + VEH groups (p > 0.05). These data show that despite significant MS ChAT+ cell loss, the EE-mediated benefit in cognitive recovery is not compromised.
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