Plasma miR-1247-5p, miR-301b-3p and miR-105-5p as potential biomarkers for early diagnosis of non-small cell lung cancer.
Plasma miR-1247-5p, miR-301b-3p and miR-105-5p as potential biomarkers for early diagnosis of non-small cell lung cancer.
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血浆 miR-1247-5p、miR-301b-3p 和 miR-105-5p 作为非小细胞肺癌早期诊断的潜在生物标志物
DOI：
10.1111/1759-7714.13800
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发表时间：
2021-03
期刊：
影响因子：
2.9
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Dong X;Chang M;Song X;Ding S;Xie L;Song X
越来越多的证据表明，microRNAs表达异常，在非小细胞肺癌(NSCLC)的发生、发展过程中发挥重要作用。应用miRNA微阵列技术对5例健康献血员和4例非小细胞肺癌患者的血浆miRNAs进行分析。对154例非小细胞肺癌患者和146例健康献血员差异表达的miRNAs进行RNA提取，并用定量聚合酶链式反应(QPCR)进行验证。通过miRNA芯片分析，筛选出40个非小细胞肺癌患者和健康献血者的差异miRNAs。我们发现，患者血浆miR-1247-5p、miR-301b-3p和miR-105-5p水平显著高于健康对照组。受试者工作特征曲线(ROC)分析显示，癌胚抗原(CEA)与miR-1247-5p、miR-301b-3p或miR-105-5p联合应用具有较高的ROC曲线下面积(AUC)和较高的敏感性/特异度。MiR-1247-5p、miR-301b-3p和miR-105-5p的高表达促进了肿瘤的发生，这三种miRNAs可作为NSCLC患者早期诊断的新的生物标志物。血浆miR-1247-5p、miR-301b-3p和miR-105-5p可作为早期NSCLC和NSCLC的新生物标志物。综上所述，我们的结果表明miR-1247-5p、miR-301b-3p和miR-105-5p是NSCLC的候选生物标志物，当它们与CEA水平相结合时表现更好。这些发现也促使我们推测，在NSCLC疾病过程中miR-1247-5p、miR-301b-3p和miR-105-5p的早期调节失调，以及只需要少量血浆，使得miR-1247-5p、miR-301b-3p和miR-105-5p非常适合作为NSCLC的早期预警生物标志物。
Accumulating evidence shows that microRNAs are aberrantly expressed and exert essential roles in the tumorigenesis and tumor progression of non‐small cell lung cancer (NSCLC). The plasma miRNAs from five healthy donors and four NSCLC patients were profiled by miRNA microarray. The differentially expressed miRNAs from 154 primary NSCLC patients and 146 healthy donors were subjected to RNA isolation and verified by quantitative PCR (qPCR). The miRNA microarray analysis revealed that 40 differential miRNAs between NSCLC patients and healthy donors were selected. We found that the plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p levels of patients were significantly higher than those of healthy controls. The receiver operating characteristic curve (ROC) analyses revealed higher area under the ROC curve (AUC) values and higher sensitivity/specificity of carcinoembryonic antigen (CEA) in combination with miR‐1247‐5p, miR‐301b‐3p, or miR‐105‐5p were superior to that of CEA alone. High miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p expression have been demonstrated to accelerate tumorigenesis, and these three miRNAs in plasma act as novel biomarkers for the early diagnosis of NSCLC patients. Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p act as novel biomarkers for early NSCLC and NSCLC. In summary, our results indicate that miR‐1247‐5p, miR‐301b‐3p, and miR‐105‐5p are candidate biomarkers of NSCLC, which perform even better when combined with CEA levels. These findings also prompted us to hypothesize that the early dysregulation of miR‐1247‐5p, miR‐301b‐3p, and miR‐105‐5p in the NSCLC disease process, and the requirement of only a small amount of plasma, render miR‐1247‐5p, miR‐301b‐3p, and miR‐105‐5p very suitable as early warning biomarkers for NSCLC.
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DOI：
10.3322/caac.21244
发表时间：
2014-09
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Berindan-Neagoe I;Monroig Pdel C;Pasculli B;Calin GA
通讯作者：
Calin GA
肿瘤衍生的外泌体 miRNA 作为非小细胞肺癌的诊断生物标志物。
影响因子：
4.7
作者：
Zhang Z;Tang Y;Song X;Xie L;Zhao S;Song X
通讯作者：
Song X
影响因子：
3.9
作者：
Wang J;Raimondo M;Guha S;Chen J;Diao L;Dong X;Wallace MB;Killary AM;Frazier ML;Woodward TA;Wang J;Sen S
通讯作者：
Sen S
DOI：
10.1007/978-3-319-23730-5_9
发表时间：
2015-01-01
期刊：
MICRORNA: CANCER: FROM MOLECULAR BIOLOGY TO CLINICAL PRACTICE
影响因子：
--
作者：
Del Vescovo, Valerio;Denti, Michela A.
通讯作者：
Denti, Michela A.
影响因子：
1.8
作者：
Shaw, Pamela A.;Pepe, Margaret S.;Etzioni, Ruth
通讯作者：
Etzioni, Ruth