Circulating microRNAs in Pancreatic Juice as Candidate Biomarkers of Pancreatic Cancer.
Circulating microRNAs in Pancreatic Juice as Candidate Biomarkers of Pancreatic Cancer.
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DOI：
10.7150/jca.10094
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发表时间：
2014
影响因子：
3.9
通讯作者：
Sen S
中科院分区：
文献类型：
--
作者：
Wang J;Raimondo M;Guha S;Chen J;Diao L;Dong X;Wallace MB;Killary AM;Frazier ML;Woodward TA;Wang J;Sen S
开发敏感和特异的生物标记物，最好是那些在体液中循环的生物标记物，对于癌症的早期诊断至关重要。本研究以6例胰腺导管腺癌(PDAC)患者的胰液和6例非胰腺、非健康(NPNH)患者的胰液为对照，采用基因芯片技术对胰腺外分泌物(胰液)中的microRNAs(MiRNAs)进行了分析。随后在来自50名PDAC、19名慢性胰腺炎(CP)患者和19名NPNH对照组的88份胰液样本中验证了差异循环miRNAs。从PDAC患者和非胰腺疾病患者的胰液中观察到四种循环miRNAs(miR-205、miR-210、miR-492和miR-1427)的图谱有显著差异。四种miRNAs水平升高联合预测PDAC的特异度为88%，灵敏度为87%。结合血清CA19-9水平可将敏感性提高到91%，特异性提高到100%。胰液中四种miRNAs的丰富与OS的降低有关，miR-205和miR-210的组合也是如此。MiR-205和miR-210的高表达也与淋巴结转移有关。因此，胰液中循环miR-205、miR-210、miR-492和miR-1247水平升高是PDAC患者疾病和预后不良的潜在候选生物标志物。
Development of sensitive and specific biomarkers, preferably those circulating in body fluids is critical for early diagnosis of cancer. This study performed profiling of microRNAs (miRNAs) in exocrine pancreatic secretions (pancreatic juice) by microarray analysis utilizing pancreatic juice from 6 pancreatic ductal adenocarcinoma (PDAC) patients and two pooled samples from 6 non-pancreatic, non-healthy (NPNH) as controls. Differentially circulating miRNAs were subsequently validated in 88 pancreatic juice samples from 50 PDAC, 19 chronic pancreatitis (CP) patients and 19 NPNH controls. A marked difference in the profiles of four circulating miRNAs (miR-205, miR-210, miR-492, and miR-1427) was observed in pancreatic juice collected from patients with PDAC and those without pancreatic disease. Elevated levels of the four miRNAs together predicted PDAC with a specificity of 88% and sensitivity of 87%. Inclusion of serum CA19-9 level increased the sensitivity to 91% and the specificity to 100%. Enrichment of the four miRNAs in pancreatic juice was associated with decreased OS, as was the combination of miR-205 and miR-210. Higher contents of miR-205 and miR-210 were also associated with lymph node metastasis. Elevated levels of circulating miR-205, miR-210, miR-492, and miR-1247 in pancreatic juice are, therefore, promising candidate biomarkers of disease and poor prognosis in patients with PDAC.
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影响因子：
6.5
作者：
Lawrie, Charles H.;Gal, Shira;Harris, Adrian L.
通讯作者：
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影响因子：
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通讯作者：
Croce, CM