The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex.
The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex.
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地中海烟粉虱复合体线粒体基因组的特征和表达谱
DOI：
10.1186/1471-2164-14-401
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发表时间：
2013-06-17
期刊：
影响因子：
4.4
通讯作者：
Liu SS
中科院分区：
文献类型：
--
作者：
Wang HL;Yang J;Boykin LM;Zhao QY;Li Q;Wang XW;Liu SS
背景：烟粉虱Bemisia tabaci（Gennadius）（粉虱科：半翅目）是一个由至少31个隐种组成的物种复合体，其中一些是全球性入侵农业害虫。此前，土著新大陆B.结果表明，烟粉虱的丝裂基因组序列与假定的烟粉虱祖先基因序列存在较大差异，而其它B的丝裂基因组序列和基因序列与假定的烟粉虱祖先基因序列和基因序列存在较大差异。烟粉虱种类不详。此外，我们还对B.结果：本研究获得了已被鉴定为B模式种的地中海入侵烟粉虱（MED）的完整有丝分裂基因组（15，632 bp）。烟粉虱复合体它编码37个基因，包括13个蛋白质编码基因（PCG）、2个核糖体RNA和22个转运RNA（tRNA）。MED和新世界（以前发表）物种的有丝分裂基因组的比较分析表明，没有基因排列。基于Illumina测序数据，分析MED有丝分裂基因组的基因表达谱。我们发现许多基因被多聚腺苷酸化，cox 1、cox 2和nd 5中的部分终止密码子通过多聚腺苷酸化完成，将T变为TAA终止密码子。此外，结合转录组和序列比对数据，确定了一些PCG可能的终止位点。我们的分析还发现，atp 6和atp 8，nd 4和nd 4l，nd 6和cytb存在于相同的顺反子转录本上，而其他成熟线粒体转录本是单顺反子的。RT-PCR分析不同发育阶段的线粒体PCGs的表达，结果表明，在若虫、蛹和成虫的各个发育阶段，线粒体PCGs基因的表达水平存在差异。有趣的是，mRNA水平显示位于同一转录单位的基因之间的显着差异，这表明线粒体mRNA丰度是严重调制的转录后regulation.Conclusions：这项工作提供了新的见解到有丝分裂基因组进化的B。结果表明，利用RNA-seq数据获取烟粉虱线粒体基因组，分析线粒体基因表达特征是可行的。
Background:The whiteflies under the name Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera) are species complex of at least 31 cryptic species some of which are globally invasive agricultural pests. Previously, the mitochondrial genome (mitogenome) of the indigenous New World B. tabaci species was sequenced and major differences of gene order from the postulated whitefly ancestral gene order were found. However, the sequence and gene order of mitogenomes in other B. tabaci species are unknown. In addition, the sequence divergences and gene expression profiles of mitogenomes in the B. tabaci species complex remain completely unexplored.Results:In this study, we obtained the complete mitogenome (15,632 bp) of the invasive Mediterranean (MED), which has been identified as the type species of the B. tabaci complex. It encodes 37 genes, including 13 protein-coding genes (PCGs), 2 ribosomal RNAs and 22 transfer RNAs (tRNA). Comparative analyses of the mitogenomes from MED and New World (previously published) species reveal that there are no gene arrangements. Based on the Illumina sequencing data, the gene expression profile of the MED mitogenome was analyzed. We found that a number of genes were polyadenylated and the partial stop codons in cox1, cox2 and nd5 are completed via polyadenylation that changed T to the TAA stop codon. In addition, combining the transcriptome with the sequence alignment data, the possible termination site of some PCGs were defined. Our analyses also revealed that atp6 and atp8, nd4 and nd4l, nd6 and cytb were found on the same cistronic transcripts, whereas the other mature mitochondrial transcripts were monocistronic. Furthermore, RT-PCR analyses of the mitochondrial PCGs expression in different developmental stages revealed that the expression level of individual mitochondrial genes varied in each developmental stage of nymph, pupa and adult. Interestingly, mRNA levels showed significant differences among genes located in the same transcription unit suggesting that mitochondrial mRNA abundance is heavily modulated by post-transcriptional regulation.Conclusions:This work provides novel insights into the mitogenome evolution of B. tabaci species and demonstrates that utilizing RNA-seq data to obtain the mitogenome and analyze mitochondrial gene expression characteristics is practical.
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影响因子：
5
作者：
Brown, JK
通讯作者：
Brown, JK
影响因子：
4.1
作者：
Cameron, Stephen L.;Lo, Nathan;Evans, Theodore A.
通讯作者：
Evans, Theodore A.
广泛的实地调查结合系统发育分析揭示了两种外来粉虱在中国的快速和广泛入侵
影响因子：
3.7
作者：
Hu, Jian;De Barro, Paul;Liu, Shu-Sheng
通讯作者：
Liu, Shu-Sheng
DOI：
10.1007/978-3-642-22380-8_10
发表时间：
2012-01-01
期刊：
ORGANELLE GENETICS: EVOLUTION OF ORGANELLE GENOMES AND GENE EXPRESSION
影响因子：
--
作者：
Barth, Christian;Kennedy, Luke A.;Fisher, Paul R.
通讯作者：
Fisher, Paul R.
影响因子：
2.6
作者：
Brehm, A;Harris, DJ;González, AM
通讯作者：
González, AM