Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers
Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers
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对遭受水浸和束缚应激的大鼠进行比较蛋白质组分析，以深入了解胃溃疡
DOI：
10.3892/mmr.2017.7241
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发表时间：
2017-08
影响因子：
3.4
通讯作者：
Ding W
中科院分区：
文献类型：
--
作者：
Zhou Zheng-Rong;Huang Pan;Song Guang-Hao;Zhang Zhuang;An Ke;Lu Han-Wen;Ju Xiao-Li;Ding Wei;Ding W
在本研究中，比较蛋白质组学分析进行了大鼠浸水束缚应激（WRS）。共26个蛋白质的差异表达，并确定使用基质辅助激光解吸/电离飞行时间质谱。在鉴定的26个差异表达蛋白点中，有13个蛋白在WRS下显著上调，包括丙酮酸激酶和钙网蛋白，这可能与能量代谢密切相关。此外，12种蛋白质在WRS下下调，包括血红蛋白亚基β-2和II型角蛋白细胞骨架蛋白8，这可能在蛋白质代谢和细胞死亡中起重要作用。基因本体分析揭示了所鉴定蛋白的细胞分布、分子功能和生物学过程。采用荧光定量聚合酶链反应分析某些差异表达蛋白的mRNA水平。本研究的结果旨在提供对应激性胃溃疡中差异表达的蛋白质的见解，并为人类胃溃疡的根治提供理论证据。
In the present study, comparative proteomic analysis was performed in rats subjected to water immersion-restraint stress (WRS). A total of 26 proteins were differentially expressed and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Among the 26 differentially expressed protein spots identified, 13 proteins were significantly upregulated under WRS, including pyruvate kinase and calreticulin, which may be closely associated with energy metabolism. In addition, 12 proteins were downregulated under WRS, including hemoglobin subunit β-2 and keratin type II cytoskeletal 8, which may be important in protein metabolism and cell death. Gene Ontology analysis revealed the cellular distribution, molecular function and biological processes of the identified proteins. The mRNA levels of certain differentially expressed proteins were analyzed using fluorescence quantitative polymerase chain reaction analysis. The results of the present study aimed to offer insights into proteins, which are differentially expressed in gastric ulcers in stress, and provide theoretical evidence of a radical cure for gastric ulcers in humans.
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影响因子：
5.6
作者：
Luiz-Ferreira A;Cola M;Barbastefano V;de-Faria FM;Almeida AB;Farias-Silva E;Calvo TR;Hiruma-Lima CA;Vilegas W;Souza-Brito AR
通讯作者：
Souza-Brito AR
影响因子：
3.7
作者：
Zeng T;Jiang X;Li J;Wang D;Li G;Lu L;Wang G
通讯作者：
Wang G
影响因子：
2.5
作者：
Kemmerly T;Kaunitz JD
通讯作者：
Kaunitz JD
影响因子：
2
作者：
Serafín-Higuera I;Garibay-Cerdenares OL;Illades-Aguiar B;Flores-Alfaro E;Jiménez-López MA;Sierra-Martínez P;Alarcón-Romero Ldel C
通讯作者：
Alarcón-Romero Ldel C
影响因子：
3.7
作者：
Mello AA;Leal MF;Rey JA;Pinto GR;Lamarão LM;Montenegro RC;Alves AP;Assumpção PP;Borges Bdo N;Smith MC;Burbano RR
通讯作者：
Burbano RR