Comparative proteomic analysis of the hepatic response to heat stress in Muscovy and Pekin ducks: insight into thermal tolerance related to energy metabolism.
Comparative proteomic analysis of the hepatic response to heat stress in Muscovy and Pekin ducks: insight into thermal tolerance related to energy metabolism.
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DOI：
10.1371/journal.pone.0076917
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Zeng T;Jiang X;Li J;Wang D;Li G;Lu L;Wang G
北京鸭是世界上最著名的肉鸭之一，由中国的绿头鸭（Anas platyrhynchos）培育而成。然而，它比被认为起源于南美洲的番鸭对热应激更敏感。随着温度的升高，北京鸭的死亡率、产蛋性能和肉质都受到严重影响。本研究旨在利用比较蛋白质组学方法揭示两种鸭的温度依赖蛋白。在39°C±0.5°C下培养1 h，然后立即返回20°C进行3 h的恢复期，提取肝脏蛋白并在二维模式下电泳。经凝胶图像分析，共检测到61个差异表达蛋白，其中54个通过MALDI TOF/TOF ms可明确鉴定。在鉴定的54个差异表达蛋白斑点中，7个在两个种属中均有发现，47个为种属特异性斑点（25个为燕尾鸭，22个为北京鸭）。众所周知，热休克蛋白（HSP） 70和HSP10等伴侣蛋白在热应激下在两种物种中都大量上调。然而，我们也发现α-烯醇化酶和s -腺苷蛋氨酸合成酶等几种蛋白质在两种鸭中表现出不同的表达模式。根据基因本体分析，将富集的生物过程分为3大类：细胞死亡与凋亡（20.93%）、氨基酸代谢（13.95%）和氧化还原（20.93%）。实时荧光定量pcr检测几种差异表达蛋白的mRNA水平。据我们所知，这项研究首次提供了对鸭子热应激后蛋白质差异表达的见解，并使我们更好地了解动物可能的热应激反应机制。
The Pekin duck, bred from the mallard (Anas platyrhynchos) in china, is one of the most famous meat duck species in the world. However, it is more sensitive to heat stress than Muscovy duck, which is believed to have originated in South America. With temperature raising, mortality, laying performance, and meat quality of the Pekin duck are severely affected. This study aims to uncover the temperature-dependent proteins of two duck species using comparative proteomic approach. Duck was cultured under 39°C ± 0.5°C for 1 h, and then immediately returned to 20°C for a 3 h recovery period, the liver proteins were extracted and electrophoresed in two-dimensional mode. After analysis of gel images, 61 differentially expressed proteins were detected, 54 were clearly identified by MALDI TOF/TOF MS. Of the 54 differentially expressed protein spots identified, 7 were found in both species, whereas 47 were species specific (25 in Muscovy duck and 22 in Pekin duck). As is well known, chaperone proteins, such as heat shock protein (HSP) 70 and HSP10, were abundantly up-regulated in both species in response to heat stress. However, we also found that several proteins, such as α-enolase, and S-adenosylmethionine synthetase, showed different expression patterns in the 2 duck species. The enriched biological processes were grouped into 3 main categories according to gene ontology analysis: cell death and apoptosis (20.93%), amino acid metabolism (13.95%) and oxidation reduction (20.93%). The mRNA levels of several differentially expressed protein were investigated by real-time RT-PCR. To our knowledge, this study is the first to provide insights into the differential expression of proteins following heat stress in ducks and enables better understanding of possible heat stress response mechanisms in animals.
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