Influence of prefoldin subunit 4 on the tolerance of Kluyveromyces marxianus to lignocellulosic biomass-derived inhibitors.
Influence of prefoldin subunit 4 on the tolerance of Kluyveromyces marxianus to lignocellulosic biomass-derived inhibitors.
复制标题
前折叠蛋白亚基 4 对马克斯克鲁维酵母对木质纤维素生物质衍生抑制剂耐受性的影响。
DOI：
10.1186/s12934-021-01715-y
复制
发表时间：
2021-12-14
影响因子：
6.4
通讯作者：
Hong J
中科院分区：
文献类型：
--
作者：
Zhang N;Shang Y;Wang F;Wang D;Hong J
马克思克鲁维酵母（Kluyveromycesmarxianus）是利用木质纤维素生物质的微生物细胞工厂的潜在优良宿主，这是由于其耐热性、高生长速率和宽底物谱。然而，其对来自木质纤维素生物质预处理的抑制剂的耐受性需要改进。前折叠蛋白复合物参与细胞骨架的折叠，与植物的抗逆性有关，而且前折叠蛋白的几个亚基已被证实参与基因表达调控。抑制剂的存在下，编码亚基4的前折叠蛋白（KmPFD 4），一个可能的转录因子的基因的表达，显着改变。因此，选择KmPFD 4来评价其在抑制剂耐受中的功能。在这项研究中，破坏前折叠蛋白亚基4基因（KmPFD 4）导致细胞内活性氧（ROS）浓度增加，并在抑制剂存在下扰乱肌动蛋白和微管蛋白的组装，导致抑制剂耐受性降低。KmPFD 4的核定位表明它可以调控基因的表达。转录组学分析表明，与ROS消除，ATP产生和NAD+合成相关的上调基因表达，这是对抑制剂存在的反应，在KmPFD 4破坏的细胞中消失。因此，KmPFD 4通过维持细胞骨架的整合并直接或间接影响响应于抑制剂的基因表达来影响抑制剂耐受性。最后，KmPFD 4的过表达增强了乙醇发酵，在抑制剂存在下生产率提高了46.27%。该研究表明KmPFD 4在抑制剂耐受性中起积极作用，可用于开发耐受抑制剂的平台菌株。在线版本包含补充材料，可通过10.1186/s12934-021-01715-y获得。
Kluyveromyces marxianus is a potentially excellent host for microbial cell factories using lignocellulosic biomass, due to its thermotolerance, high growth rate, and wide substrate spectrum. However, its tolerance to inhibitors derived from lignocellulosic biomass pretreatment needs to be improved. The prefoldin complex assists the folding of cytoskeleton which relates to the stress tolerance, moreover, several subunits of prefoldin have been verified to be involved in gene expression regulation. With the presence of inhibitors, the expression of a gene coding the subunit 4 of prefoldin (KmPFD4), a possible transcription factor, was significantly changed. Therefore, KmPFD4 was selected to evaluate its functions in inhibitors tolerance. In this study, the disruption of the prefoldin subunit 4 gene (KmPFD4) led to increased concentration of intracellular reactive oxygen species (ROS) and disturbed the assembly of actin and tubulin in the presence of inhibitors, resulting in reduced inhibitor tolerance. Nuclear localization of KmPFD4 indicated that it could regulate gene expression. Transcriptomic analysis showed that upregulated gene expression related to ROS elimination, ATP production, and NAD+ synthesis, which is a response to the presence of inhibitors, disappeared in KmPFD4-disrupted cells. Thus, KmPFD4 impacts inhibitor tolerance by maintaining integration of the cytoskeleton and directly or indirectly affecting the expression of genes in response to inhibitors. Finally, overexpression of KmPFD4 enhanced ethanol fermentation with a 46.27% improvement in productivity in presence of the inhibitors. This study demonstrated that KmPFD4 plays a positive role in the inhibitor tolerance and can be applied for the development of inhibitor-tolerant platform strains. The online version contains supplementary material available at 10.1186/s12934-021-01715-y.
登录
查看更多内容
影响因子：
6.3
作者：
Allen SA;Clark W;McCaffery JM;Cai Z;Lanctot A;Slininger PJ;Liu ZL;Gorsich SW
通讯作者：
Gorsich SW
影响因子：
4.1
作者：
BANAT, IM;NIGAM, P;MARCHANT, R
通讯作者：
MARCHANT, R
影响因子：
3.8
作者：
Kim, Sang Yong;Kim, Jin Chul;Ahn, Jeong Keun
通讯作者：
Ahn, Jeong Keun
苯酚、糠醛和乙酸对酿酒酵母相互作用影响的代谢组学研究
影响因子：
3.3
作者：
Ding, Ming-Zhu;Wang, Xin;Yuan, Ying-Jin
通讯作者：
Yuan, Ying-Jin
影响因子：
3.8
作者：
Jo, William J.;Loguinov, Alex;Vulpe, Chris D.
通讯作者：
Vulpe, Chris D.