Standardized rice bran extract improves hepatic steatosis in HepG2 cells and ovariectomized rats.
Standardized rice bran extract improves hepatic steatosis in HepG2 cells and ovariectomized rats.
复制标题
DOI：
10.4162/nrp.2020.14.6.568
复制
发表时间：
2020-12
影响因子：
2.4
通讯作者：
Um MY
中科院分区：
文献类型：
--
作者：
Lim DW;Jeon H;Kim M;Yoon M;Jung J;Kwon S;Cho S;Um MY
肝脏脂肪变性是最常见的肝脏疾病，尤其是在绝经后的女性。本研究探讨标准化米糠提取物(RBS)对去卵巢(OVX)诱导的大鼠肝脏脂肪变性的保护作用。用2 0 0µM油酸与RBS和γ谷维素共同孵育细胞，诱导脂质蓄积。将去卵巢大鼠分成3组，分别饲喂正常饲料(ND)和含17β-雌二醇(E_2，10µg/kg)和RBS(500 mg/kg)的饲料16周。补充RBS可改善去卵巢大鼠的血清甘油三酯和游离脂肪酸水平。组织学分析显示，RBS显著减少肝脏脂肪堆积，降低肝脏脂肪、总胆固醇和甘油三酯水平。此外，RBS还抑制雌激素缺乏诱导的成脂基因的上调，如固醇调节元件结合蛋白1、乙酰辅酶A羧基酶1、脂肪酸合成酶、3-磷酸甘油酰基转移酶和硬脂酰辅酶A去饱和酶1。RBS和γ-谷维素有效地减少了肝脏脂肪变性模型中的脂质积聚。RBS通过调节SREBP1介导的造脂基因的激活来改善OVX诱导的肝脏脂肪变性，表明RBS在预防绝经后女性脂肪肝方面的好处。
Hepatic steatosis is the most common liver disorder, particularly in postmenopausal women. This study investigated the protective effects of standardized rice bran extract (RBS) on ovariectomized (OVX)-induced hepatic steatosis in rats. HepG2 cells were incubated with 200 µM oleic acid to induce lipid accumulation with or without RBS and γ-oryzanol. OVX rats were separated into three groups and fed a normal diet (ND) or the ND containing 17β-estradiol (E2; 10 µg/kg) and RBS (500 mg/kg) for 16 weeks. RBS supplementation improved serum triglyceride and free fatty acid levels in OVX rats. Histological analysis showed that RBS significantly attenuated hepatic fat accumulation and decreased hepatic lipid, total cholesterol, and triglyceride levels. Additionally, RBS suppressed the estrogen deficiency-induced upregulation of lipogenic genes, such as sterol regulatory element-binding protein 1 (SREBP1), acetyl-CoA carboxylase 1, fatty acid synthase, glycerol-3-phosphate acyltransferase, and stearoyl-CoA desaturase 1. RBS and γ-oryzanol effectively reduced lipid accumulation in a HepG2 cell hepatic steatosis model. RBS improves OVX-induced hepatic steatosis by regulating the SREBP1-mediated activation of lipogenic genes, suggesting the benefits of RBS in preventing fatty liver in postmenopausal women.
登录
查看更多内容
影响因子：
2.2
作者：
Lavoie JM;Pighon A
通讯作者：
Pighon A
影响因子：
--
作者：
Chong, C. L. G.;Hussan, Farida;Othman, Faizah
通讯作者：
Othman, Faizah
影响因子：
5.9
作者：
Mastinu, Andrea;Bonini, Sara Anna;Uberti, Daniela
通讯作者：
Uberti, Daniela
影响因子：
2.8
作者：
Koshy, S. M.;Bobby, Z.;Paulose, D. T.
通讯作者：
Paulose, D. T.
影响因子：
5.9
作者：
Jung TD;Shin GH;Kim JM;Choi SI;Lee JH;Lee SJ;Park SJ;Woo KS;Oh SK;Lee OH
通讯作者：
Lee OH