Genome Wide Identification and Expression Profiling of SWEET Genes Family Reveals Its Role During Plasmodiophora brassicae-Induced Formation of Clubroot in Brassica rapa.
Genome Wide Identification and Expression Profiling of SWEET Genes Family Reveals Its Role During Plasmodiophora brassicae-Induced Formation of Clubroot in Brassica rapa.
复制标题
SWEET 基因家族的全基因组鉴定和表达谱揭示了其在甘蓝根肿菌诱导的白菜根肿病形成过程中的作用
DOI：
10.3389/fpls.2018.00207
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Li H;Li X;Xuan Y;Jiang J;Wei Y;Piao Z
芸苔Plasmodiophora brassicae是一种土传病原菌，是造成芸苔类作物根腐病的病原。这种病原体生活在根部，并从寄主植物中获取营养。这表明球茎溃疡病在受感染的根中产生了额外的营养汇。然而，芸苔菌感染和糖转运的分子机制尚不清楚。在此，我们利用一对白菜近等基因系（NILs）分析了甘蓝侵染前和侵染后叶片和根系的糖含量，这些系在CRb位点携带一个根茎抗性（CR）或易感（CS）等位基因。与CR-NIL相比，受brassicae侵染后CS-NIL植株根部葡萄糖和果糖含量显著增加，说明糖转运与brassicae生长密切相关。在32个rapa SWEET同源物中，属于ⅰ和ⅲ支系的多个BrSWEETs在brassicae感染后显著上调，尤其是CS-NIL。此外，与野生型植株相比，拟南芥sweet11突变体的胆囊形成速度较慢。我们的研究表明，芸苔菌感染可能引发产糖组织和棒状组织之间活跃的糖易位，而SWEET家族基因参与了这一过程。
Plasmodiophora brassicae is a soil borne pathogen and the causal agent of clubroot, a devastating disease of Brassica crops. The pathogen lives inside roots, and hijacks nutrients from the host plants. It is suggested that clubroot galls created an additional nutrient sink in infected roots. However, the molecular mechanism underlying P. brassicae infection and sugar transport is unclear. Here, we analyzed sugar contents in leaves and roots before and after P. brassicae infection using a pair of Chinese cabbage near-isogenic lines (NILs), carrying either a clubroot resistant (CR) or susceptible (CS) allele at the CRb locus. P. brassicae infection caused significant increase of glucose and fructose contents in the root of CS-NIL compared to CR-NIL, suggesting that sugar translocation and P. brassicae growth are closely related. Among 32 B. rapa SWEET homologs, several BrSWEETs belonging to Clade I and III were significantly up-regulated, especially in CS-NIL upon P. brassicae infection. Moreover, Arabidopsis sweet11 mutant exhibited slower gall formation compared to the wild-type plants. Our studies suggest that P. brassicae infection probably triggers active sugar translocation between the sugar producing tissues and the clubbed tissues, and the SWEET family genes are involved in this process.
登录
查看更多内容
影响因子：
4.4
作者：
Irani S;Trost B;Waldner M;Nayidu N;Tu J;Kusalik AJ;Todd CD;Wei Y;Bonham-Smith PC
通讯作者：
Bonham-Smith PC
影响因子：
10.7
作者：
Pineau, P;Henry, M;Vartanian, JP
通讯作者：
Vartanian, JP
影响因子：
3.2
作者：
Faggian, R;Bulman, SR;Porter, IJ
通讯作者：
Porter, IJ
影响因子：
10.5
作者：
Chu, Zhaohui;Yuan, Meng;Wang, Shiping
通讯作者：
Wang, Shiping
影响因子：
2.6
作者：
Artero, RD;Terol-Alcayde, J;Perez-Alonso, M
通讯作者：
Perez-Alonso, M