Nosema ceranae infection enhances Bifidobacterium spp. abundances in the honey bee hindgut
Nosema ceranae infection enhances Bifidobacterium spp. abundances in the honey bee hindgut
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蜜蜂微孢子虫感染可增强双歧杆菌属。
DOI：
10.1007/s13592-019-00644-5
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发表时间：
2019-07
期刊：
影响因子：
2.4
通讯作者：
Huang Wei-Fone
中科院分区：
文献类型：
--
作者：
Zhang Yakun;Lu Xiaoxiao;Huang Shaokang;Zhang Lina;Su Songkun;Huang Wei-Fone
蜜蜂微孢子虫已成为蜜蜂 (Apis mellifera) 中的主要微孢子虫病原体。它主要在中肠中感染和繁殖，繁殖后的蜜蜂孢子传播到后肠并给宿主留下粪便。后肠为细菌共生体和生物膜提供结构支持，其中含有整个蜜蜂中最密集的细菌。尽管在实验室试验中一些细菌对中华脉络膜炎有抑制作用，但后肠细菌并没有明显阻碍中华脉络膜炎疾病。我们怀疑神经杆菌感染可能会影响后肠细菌种群，从而有利于孢子的存活。在这项研究中，使用焦磷酸测序方法分析了排便的觅食者的后肠细菌微生物组。结果表明双歧杆菌属。丰度与 N. ceranae 感染呈显着正相关。为了在更大的样本量中证实这种关联，我们使用笼养蜜蜂和 qPCR 来量化双歧杆菌和乳酸菌。在排空的后肠样本中也发现了相同的关联，但在粪便样本中并不显着。当后肠上的双歧杆菌因蜜蜂感染而显着增强时，粪便中的双歧杆菌可能会增加。尽管我们尚未确定这种关联的具体原因和机制，但结果表明本土双歧杆菌属。蜜蜂后肠中的细菌可能对蜜蜂疫病没有预防作用。
Nosema ceranae has become the dominant Nosema pathogen in the honey bees, Apis mellifera. It primarily infects and reproduces in midguts and reproduced N. ceranae spores travel to hindguts and leave hosts with feces. Hindguts have structural support for bacterial symbionts and biofilms, which harbor the densest bacteria of the entire bee. Although some bacteria were inhibitory to N. ceranae in the laboratory trials, N. ceranae disease is not apparently hurdled by hindgut bacteria. We suspected N. ceranae infection may affect hindgut bacteria populations to favor the spore survival. In this study, hindgut bacterial microbiomes of defecated foragers were analyzed using a pyrosequencing method. The results suggested Bifidobacterium spp. abundances were significantly and positively associated with N. ceranae infection. To confirm the association in a larger sample size, we used caged bees and qPCRs that quantify bifidobacteria and lactobacteria. The same association was found in emptied hindgut samples, but not significant in feces samples. Bifidobacteria in feces might increase when bifidobacteria on hindguts are significantly enhanced by N. ceranae infection. Although we have not identified the specific causes and mechanisms of this association, the results suggested that indigenous Bifidobacterium spp. in honey bee hindguts may have no prevention effects on N. ceranae disease.
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影响因子：
9.8
作者：
Esvelt KM;Gemmell NJ
通讯作者：
Gemmell NJ
影响因子：
3.4
作者：
É. Teixeira;L. G. D. Santos;A. Sattler;D. Message;M. L. T. M. F. Alves-M.-L.-T.-M.-F.-Alves-39893553;M. Martins;Marina Lopes Grassi-Sella;T. Francoy
通讯作者：
É. Teixeira;L. G. D. Santos;A. Sattler;D. Message;M. L. T. M. F. Alves-M.-L.-T.-M.-F.-Alves-39893553;M. Martins;Marina Lopes Grassi-Sella;T. Francoy
DOI：
--
发表时间：
2008
期刊：
Chinese Bulletin of Entomology
影响因子：
--
作者：
Dai Ping
通讯作者：
Dai Ping
影响因子：
1.9
作者：
R. Paxton
通讯作者：
R. Paxton
影响因子：
5.4
作者：
Baffoni, L.;Gaggia, F.;Di Gioia, D.
通讯作者：
Di Gioia, D.