RNA sequence analysis of dermal papilla cells' regeneration in 3D culture.
RNA sequence analysis of dermal papilla cells' regeneration in 3D culture.
复制标题
3D 培养中真皮乳头细胞再生的 RNA 序列分析
DOI：
10.1111/jcmm.15965
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发表时间：
2020-11
影响因子：
5.3
通讯作者：
Lin B
中科院分区：
文献类型：
--
作者：
Lin G;Yin G;Ye J;Pan X;Zhu J;Lin B
毛乳头细胞（dermal papilla cells，DPC）是毛囊生长和再生的重要细胞。而毛乳头细胞在二维培养中经过多次传代后，其再生能力逐渐丧失。与2D培养的DPC相比，3D培养的DPC可以广泛传代。然而，DPC在三维培养中再生的分子机制仍不清楚。因此，建议采用基因测序的方法来研究二维和三维培养中毛再生的情况，建立了三组毛再生细胞，即二维培养中第2代毛再生细胞、二维培养中第8代毛再生细胞和三维培养中第8代毛再生细胞。利用维恩图对这3组基因进行了差异表达基因（DEG）的鉴定，其中已知基因1642个，新基因359个。总共1642个已知基因分别用于基因本体（GO）、京都基因、基因组百科全书（KEGG）途径富集和蛋白质-蛋白质相互作用（PPI）分析。利用Cytoscape软件对DEG的功能和作用途径进行了分析，确定了DEG的关键模块和前10位枢纽基因（IL 1B、CXCL 12、HGF、EGFR、APP、CCL 2、PTGS 2、MMP 9、NGF和SPP 1）。此外，三组的qRT-PCR结果验证了枢纽基因对毛发生长至关重要。总之，本研究所鉴定的10个中枢基因及其相关通路可用于了解毛发生长的分子机制，为毛发再生提供可能。
It is well known that dermal papilla cells (DPCs) are crucial for hair follicle growth and regeneration. However, dermal papilla cells in 2D culture could lose their ability of regeneration after several passage intervals. As opposed to DPCs in 2D culture, the DPCs in 3D culture could passage extensively. However, the molecular mechanisms of DPCs’ regeneration in 3D culture remain unclear. Accordingly, gene sequencing is recommended for the investigation of hair regeneration between 2D and 3D culture, the three groups were established including DPCs in passage 2 in 2D culture, DPCs in passage 8 in 2D culture and DPCs in passage 8 in 3D culture. The differentially expressed genes (DEGs) were identified using the Venn diagram of these three groups, which included 1642 known and 359 novel genes, respectively. A total of 1642 known genes were used for Gene Ontology (GO), Kyoto Gene, Genomic Encyclopedia (KEGG) pathway enrichment and protein‐protein interaction (PPI) analyses, respectively. The functions and pathways of DEGs were enriched in biological regulation, signal transduction and immune system, etc. The key module and the top 10 hub genes (IL1B, CXCL12, HGF, EGFR, APP, CCL2, PTGS2, MMP9, NGF and SPP1) were also identified using the Cytoscape application. Furthermore, the qRT‐PCR results of the three groups validated that the hub genes were crucial for hair growth. In conclusion, the ten identified hub genes and related pathways in the current study can be used to understand the molecular mechanism of hair growth, and those provided a possibility for hair regeneration.
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影响因子：
48
作者：
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通讯作者：
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影响因子：
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
12.3
作者：
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通讯作者：
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