Assessment of neutralizing antibody responses after natural SARS-CoV-2 infection and vaccination in congolese individuals.
Assessment of neutralizing antibody responses after natural SARS-CoV-2 infection and vaccination in congolese individuals.
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DOI：
10.1186/s12879-022-07593-y
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发表时间：
2022-07-13
影响因子：
3.7
通讯作者：
Ntoumi, Francine
中科院分区：
文献类型：
--
作者：
Batchi-Bouyou, Armel Landry;Djontu, Jean Claude;Vouvoungui, Jeannhey Christevy;Mfoutou Mapanguy, Claujens Chastel;Lobaloba Ingoba, Line;Mougany, Jire Sephora;Boumpoutou, Kamal Rauchelvy;Diafouka-kietela, Steve;Ampa, Raoul;Ntoumi, Francine
评估接种疫苗后的免疫应答是真实的世界中疫苗有效性评估包的一部分。关于SARS-CoV-2，中和抗体水平已被证明是抗体免疫应答增强的良好指标。迄今为止，非洲包括中部非洲报告的数据有限。本研究的目的是提供B.1.214.1和B.1.617.2变异波期间接种和未接种疫苗（包括自然感染的刚果人群）的抗S1加标总IgG和中和抗体数据。招募的患者分为4组：（1）2021年1月自然感染B.1.214.1变异株，随访至2021年9月。这些患者已在07个月接种疫苗，然后在接种疫苗后随访2个月;（2）自2021年6月起自然感染B.1.617.2变异株;（3）未接种SARS-CoV-2疫苗且无既往SARS-CoV-2感染史的个体;（4）完全接种国药集团/BBIP-CorV或杨森/Ad26.COV2.S疫苗的个体。通过qRT-PCR检测SARS-CoV-2，并使用下一代测序仪进行测序。ELISA法检测IgG，中和抗体采用商业中和试验。感染B.1214.1变异体的个体在02、03和06个月时引起一致的高IgG滴度。BBIP-CorV疫苗接种后2个月，受试者显示总IgG显著增加2.5倍（p < 0.0001），中和抗体能力显著增加1.5倍。这项研究表明，与B1.214.2变异体感染相比，B1.617.2（delta）变异体的自然感染更具免疫原性。我们发现，与未接种疫苗的参与者相比，完全接种疫苗的参与者的抗SARS-CoV-2 IgG浓度（p < 0.0002）和抗体中和能力（P < 0.0001）显著更高。接种后2个月，与BBIP-CorV相比，接受Janssen/Ad26.COV2.S的个体呈现出更高（p = 0.01）的总IgG/刺突蛋白。在刚果人群中，自然感染和BBIP-CorV和Janssen/Ad26.COV2.S疫苗接种均诱导抗体应答。此外，Janssen/Ad26.COV2.S的免疫原性高于Sinopharm/BBIP-CorV。有必要调查这些抗体在先前感染和未接种疫苗的刚果人中的持续时间，以使公共卫生利益攸关方能够就刚果人口的疫苗接种时间表做出基于证据的决定。
Assessing immune responses after vaccination is part of the evaluation package of vaccine effectiveness in the real world. Regarding SARS-CoV-2, neutralizing antibody levels has been shown to be a good indicator of antibody immune response boosting. So far, limited data have been reported from Africa including in Central Africa. The objective of this study was to provide data on anti-S1 spike total IgG and neutralizing antibodies in vaccinated and non-vaccinated including naturally infected Congolese population during B.1.214.1 and B.1.617.2 variant waves. Recruited patients were divided into 4 groups: (1) Naturally infected by the B.1.214.1 variant on January 2021 and followed up until September 2021. These patients have been vaccinated at month 07 and then followed up for 2 months post vaccination; (2) Naturally infected by the B.1.617.2 variant from June 2021; (3) unvaccinated SARS-CoV-2 individuals with no history of prior SARS-CoV-2 infection; (4) fully vaccinated individuals with sinopharm/BBIP-CorV or Janssen/Ad26.COV2.S. SARS-CoV-2 was detected by qRT-PCR and sequenced using Next-Generation Sequencing. ELISA method was used for detecting IgG, and neutralizing Antibody against SARS-CoV-2 antigens using commercial neutralizing assay. Individuals infected by the B.1214.1 variant elicited consistently high IgG titers at 02, 03 and 06 months. Two months post vaccination with BBIP-CorV, participants showed a significant increase by × 2.5 fold (p < 0.0001) of total IgG and X1.5 fold for neutralizing antibody capacity. This study showed that natural infection with B1.617.2 (delta) variant was more immunogenic compared to those being infected with B1.214.2 variant. We found a significantly higher concentration in anti-SARS-CoV-2 IgG (p < 0.0002) and antibodies neutralization capacity (P < 0.0001) in fully vaccinated compared to unvaccinated participants. Two months post vaccination, individuals who received Janssen/Ad26.COV2.S presented higher (p = 0.01) total IgG to spike protein compared to BBIP-CorV. Both natural infection and vaccination with BBIP-CorV and Janssen/Ad26.COV2.S induced antibody response in Congolese population. In addition, Janssen/Ad26.COV2.S was more immunogenic than Sinopharm/BBIP-CorV. There is a need to investigate the duration of these antibodies both in previously infected and naive vaccinated Congolese to allow public heath stakeholders to make evidence-based decision on vaccine schedule for the Congolese population.
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DOI：
10.1016/s2666-5247(21)00034-3
发表时间：
2021-03
期刊：
The Lancet. Microbe
影响因子：
--
作者：
Alkandari D;Herbert JA;Alkhalaf MA;Yates C;Panagiotou S
通讯作者：
Panagiotou S
影响因子：
7.8
作者：
Hueda-Zavaleta, Miguel;Gomez de la Torre, Juan C.;Alonso Caceres-Del Aguila, Jose;Muro-Rojo, Cecilia;De la Cruz-Escurra, Nathalia;Arenas Siles, Daniella;Minchon-Vizconde, Diana;Copaja-Corzo, Cesar;Bardales-Silva, Fabrizzio;Benites-Zapata, Vicente A.;Rodriguez-Morales, Alfonso J.
通讯作者：
Rodriguez-Morales, Alfonso J.
影响因子：
9.4
作者：
Addetia A;Crawford KHD;Dingens A;Zhu H;Roychoudhury P;Huang ML;Jerome KR;Bloom JD;Greninger AL
通讯作者：
Greninger AL
影响因子：
56.9
作者：
Cai, Yongfei;Zhang, Jun;Chen, Bing
通讯作者：
Chen, Bing
影响因子：
6.4
作者：
Kim, Namhee;Shin, Sue;Park, Hyunwoong
通讯作者：
Park, Hyunwoong