Dielectrophoretic capture of E. coli cells at micropatterned nanoelectrode arrays.
Dielectrophoretic capture of E. coli cells at micropatterned nanoelectrode arrays.
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DOI：
10.1002/elps.201100020
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发表时间：
2011-09
期刊：
影响因子：
2.9
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Syed, Lateef U.;Liu, Jianwei;Price, Alex K.;Li, Yi-fen;Culbertson, Christopher T.;Li, Jun
关键词：
本文报道了基于交流介电电泳（DEP）和电化学阻抗谱（EIS）的病原菌捕获和检测方法，分别采用嵌入式垂直排列碳纳米管（VACNF）纳米电极阵列（NEA）和宏观的氧化铟锡（ITO）透明电极“点-盖”结构。纳米介电电泳装置是利用微影制程在随机分布的NEA上定义一个暴露的活性区，并在ITO上定义一个微流控通道，以引导标记的E.大肠杆菌细胞，然后将它们结合到流体芯片中。施加高频（100 kHz）AC场以在暴露的CNF的尖端处产生正DEP。由于电极尺寸减小到纳米级，从而有助于克服E.大肠杆菌细胞在高流速（高达1.6毫米/秒）。这种DEP装置能够有效地捕获大量的E.杆菌绝对阻抗的显著降低（|Z|）在NEA处的电化学阻抗谱（EIS）实验中观察到。在这项研究中获得的结果表明，一个功能齐全的电子设备的快速，可逆和无标记的捕获和检测病原菌的集成的可能性。
This paper reports capture and detection of pathogenic bacteria based on AC dielectrophoresis (DEP) and electrochemical impedance spectroscopy (EIS) employing an embedded vertically aligned carbon nanofiber (VACNF) nanoelectrode array (NEA) vs. a macroscopic indium tin oxide (ITO) transparent electrode in “points-and-lid” configuration. The nano-DEP device was fabricated using photolithography processes to define an exposed active region on a randomly distributed NEA and a microfluidic channel on ITO to guide the flow of labeled E. coli cells, respectively, and then bond them into a fluidic chip. A high frequency (100 kHz) AC field was applied to generate positive DEP at the tips of exposed CNFs. Enhanced electric field gradient was achieved due to reduction in electrode size down to nanometer scale which helped to overcome the large hydrodynamic drag force experienced by E. coli cells at high flow velocities (up to 1.6 mm/sec). This DEP device was able to effectively capture a significant number of E. coli. Significant decrease in the absolute impedance (|Z|) at the NEA was observed by EIS experiments. The results obtained in this study suggest the possibility of integration of a fully functional electronic device for rapid, reversible and label-free capture and detection of pathogenic bacteria.
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