An autophagy-associated Atg8 protein is involved in the responses of Arabidopsis seedlings to hormonal controls and abiotic stresses.
An autophagy-associated Atg8 protein is involved in the responses of Arabidopsis seedlings to hormonal controls and abiotic stresses.
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DOI：
10.1093/jxb/ern244
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发表时间：
2008
影响因子：
6.9
通讯作者：
Galili G
中科院分区：
文献类型：
--
作者：
Slavikova S;Ufaz S;Avin-Wittenberg T;Levanony H;Galili G
真核生物含有普遍存在的自噬相关 Atg8 蛋白家族。在动物细胞中，这些蛋白质具有与生长、癌症和退行性疾病相关的多种功能，但它们在植物中的功能仍然很大程度上未知。为了寻找 Atg8 在植物中的新功能，本报告测试了重组 AtAtg8 蛋白的表达对拟南芥植物对细胞分裂素和生长素激素以及盐和渗透胁迫的响应的影响，该蛋白的 N 末端与绿色荧光蛋白 (GFP) 融合，C 末端与血凝素表位标签融合。 AtAtg8 融合蛋白的表达调节细胞分裂素对根结构的影响。此外，这种融合蛋白的表达还减少了响应细胞分裂素向根部供给的芽花青素积累，这意味着 AtAtg8 参与了细胞分裂素调节的根-芽通讯。细胞分裂素的外部应用导致位于根血管系统附近的细胞中形成新型包含 GFP-AtAtg8 的结构，其大小和动态运动与在根表皮细胞中观察到的包含 GFP-AtAtg8 的自噬体相似结构明显不同。 AtAtg8融合构建体的表达还使得植物对轻度盐胁迫更加敏感，并且对轻度渗透胁迫更敏感。这种敏感性还与根结构的各种变化有关，这些变化在形态上与细胞分裂素反应中观察到的变化不同。结果表明 AtAtg8 在不同的根组织中具有多种功能，这些功能也可能受到不同机制的调节。
Eukaryotes contain a ubiquitous family of autophagy-associated Atg8 proteins. In animal cells, these proteins have multiple functions associated with growth, cancer, and degenerative diseases, but their functions in plants are still largely unknown. To search for novel functions of Atg8 in plants, the present report tested the effect of expression of a recombinant AtAtg8 protein, fused at its N-terminus to green fluorescent protein (GFP) and at its C-terminus to the haemagglutinin epitope tag, on the response of Arabidopsis thaliana plants to the hormones cytokinin and auxin as well as to salt and osmotic stresses. Expression of this AtAtg8 fusion protein modulates the effect of cytokinin on root architecture. Moreover, expression of this fusion protein also reduces shoot anthocyanin accumulation in response to cytokinin feeding to the roots, implying the participation of AtAtg8 in cytokinin-regulated root–shoot communication. External application of cytokinin leads to the formation of novel GFP–AtAtg8-containing structures in cells located in the vicinity of the root vascular system, which are clearly distinct in size and dynamic movement from the GFP–AtAtg8-containing autophagosome-resembling structures that were observed in root epidermis cells. Expression of the AtAtg8 fusion construct also renders the plants more sensitive to a mild salt stress and to a lesser extent to a mild osmotic stress. This sensitivity is also associated with various changes in the root architecture, which are morphologically distinct from those observed in response to cytokinin. The results imply multiple functions for AtAtg8 in different root tissues that may also be regulated by different mechanisms.
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影响因子：
64.5
作者：
Geldner, N;Anders, N;Jürgens, G
通讯作者：
Jürgens, G
影响因子：
7.4
作者：
Hanaoka, H;Noda, T;Ohsumi, Y
通讯作者：
Ohsumi, Y
影响因子：
3.5
作者：
Ketelaar, T;Voss, C;Hussey, PJ
通讯作者：
Hussey, PJ
影响因子：
7.4
作者：
DEIKMAN, J;HAMMER, PE
通讯作者：
HAMMER, PE
影响因子：
56.9
作者：
GAN, SS;AMASINO, RM
通讯作者：
AMASINO, RM