Diagnostic efficacy of real-time PCR for ocular cytomegalovirus infections.
Diagnostic efficacy of real-time PCR for ocular cytomegalovirus infections.
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DOI：
10.1007/s00417-018-4111-9
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Real-time PCR for ocular cytomegalovirus infection study group
中科院分区：
文献类型：
--
作者：
Miyazaki D;Shimizu D;Shimizu Y;Inoue Y;Inoue T;Higaki S;Ueta M;Sugita S;Real-time PCR for ocular cytomegalovirus infection study group
本研究的目的是确定定量实时PCR（qPCR）诊断眼部巨细胞病毒（CMV）感染的有效性和临床特征。使用WHO巨细胞病毒国际标准，通过日本5家机构的qPCR检测结果评估技术因素。通过检查连续197例疑似CMV患者的197只眼的房水样本，使用受试者操作特征（ROC）评估临床因素。所有机构的检测效果都很好，尽管拷贝数范围为0.82 - 4.66拷贝/IU。在临床标本中，有51只眼睛检测到CMV，CMV视网膜炎的CMV DNA含量明显较高。在角膜疾病中，CMV DNA的量与复发频率和IOP升高显著相关。qPCR诊断的敏感性和特异性分别为90.0%和98.7%。对于角膜和前葡萄膜炎类型的CMV疾病，qPCR的曲线下面积（AUC）为0.95和0.96，其次是复发频率（AUC为0.89和0.82）和IOP升高（AUC为0.78和0.76）。未分类的巨细胞病毒检测，不符合CMV角膜内皮炎，前葡萄膜炎或视网膜炎的诊断标准，是4.6%，它与角膜疾病和角膜移植史显着相关。标准化qPCR具有特异性和准确性;然而，纳入和了解临床特征可提高诊断效力。
The aim of this study is to determine the efficacy of quantitative real-time PCR (qPCR) and clinical characteristics to diagnose ocular cytomegalovirus (CMV) infections. The technical factors were assessed by the outcomes of the qPCR assay at five institutions in Japan using the WHO International Standard of cytomegalovirus. The clinical factors were assessed by examining the aqueous humor samples of 197 eyes of 197 consecutive patients suspected of CMV using the receiver operating characteristics (ROCs). All of the institutions had excellent detection efficacy, although the copy number ranged from 0.82 to 4.66 copies/IU. In the clinical samples, CMV was detected in 51 eyes, and the amount of CMV DNA was significantly higher for CMV retinitis. In corneal diseases, the amount of CMV DNA was significantly associated with frequency of recurrences and IOP elevations. The sensitivity and specificity of qPCR for the diagnosis was 90.0 and 98.7%, respectively. For the corneal and anterior uveitis types of CMV diseases, the area under the curve (AUC) of qPCR was 0.95 and 0.96, followed by frequency of recurrences with AUC of 0.89 and 0.82, and IOP elevations with AUC of 0.78 and 0.76. Unclassified cytomegalovirus detection, which did not meet diagnostic criteria of CMV corneal endotheliitis, anterior uveitis, or retinitis, was 4.6%, and it was significantly associated with corneal diseases and history of corneal transplantation. qPCR with standardization is specific and accurate; however, the inclusion and knowledge of the clinical characteristics improve the diagnostic efficacy.
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DOI：
10.1007/s00417-010-1510-y
发表时间：
2011-01-01
影响因子：
2.7
作者：
Hwang, Yih-Shiou;Shen, Chia-Rui;Hsiao, Ching-Hsi
通讯作者：
Hsiao, Ching-Hsi
影响因子：
13.7
作者：
Jabs, DA;Van Natta, ML;Li, HK
通讯作者：
Li, HK
DOI：
10.1136/bjophthalmol-2013-304625
发表时间：
2015-01
期刊：
The British journal of ophthalmology
影响因子：
--
作者：
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通讯作者：
Japan Corneal Endotheliitis Study Group
影响因子：
13.7
作者：
Tran, Kimberly D.;Falcone, Michelle M.;Galor, Anat
通讯作者：
Galor, Anat
影响因子：
12.7
作者：
Oka, Naoko;Suzuki, Takashi;Ohashi, Yuichi
通讯作者：
Ohashi, Yuichi