Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin.
Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin.
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DOI：
10.1016/j.ydbio.2013.08.010
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发表时间：
2014-01-15
影响因子：
2.7
通讯作者：
Yablonka-Reuveni, Zipora
中科院分区：
文献类型：
--
作者：
Stuelsatz, Pascal;Shearer, Andrew;Yablonka-Reuveni, Zipora
关键词：
Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been using flow cytometry in combination with myogenic-specific Cre-loxP lineage marking for cell isolation. While analyzing cells from the EOM of mice that harbor Myf5Cre-driven GFP expression, we identified in addition to the expected GFP+ myogenic cells (presumably satellite cells), a second dominant GFP+ population distinguished as being Sca1+, non-myogenic, and exhibiting a fibro/adipogenic potential. This unexpected population was not only unique to EOM compared to the other muscles but also specific to the Myf5Cre-driven reporter when compared to the MyoDCre driver. Histological studies of periocular tissue preparations demonstrated the presence of Myf5Cre-driven GFP+ cells in connective tissue locations adjacent to the muscle masses, including cells in the vasculature wall. These vasculature-associated GFP+ cells were further identified as mural cells based on the presence of the specific XLacZ4 transgene. Unlike the EOM satellite cells that originate from a Pax3-negative lineage, these non-myogenic Myf5Cre-driven GFP+ cells appear to be related to cells of a Pax3-expressing origin, presumably derived from the neural crest. In all, our lineage tracing based on multiple reporter lines has demonstrated that regardless of common ancestral expression of Myf5, there is a clear distinction between periocular myogenic and non-myogenic cell lineages according to their mutually exclusive antecedence of MyoD and Pax3 gene activity.
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DOI：
10.1242/dev.002642
发表时间：
2007-06
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Billon N;Iannarelli P;Monteiro MC;Glavieux-Pardanaud C;Richardson WD;Kessaris N;Dani C;Dupin E
通讯作者：
Dupin E
影响因子：
2.7
作者：
Biressi, Stefano;Bjornson, Christopher R. R.;Carlig, Poppy M. M.;Nishijo, Koichi;Keller, Charles;Rando, Thomas A.
通讯作者：
Rando, Thomas A.
影响因子：
2.7
作者：
Day, Kenneth;Shefer, Gabi;Yablonka-Reuveni, Zipora
通讯作者：
Yablonka-Reuveni, Zipora
影响因子：
4.8
作者：
Billon, Nathalie;Dani, Christian
通讯作者：
Dani, Christian
影响因子：
4.6
作者：
Gensch, Nicole;Borchardt, Thilo;Braun, Thomas
通讯作者：
Braun, Thomas