Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression.
Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression.
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DOI：
10.1371/journal.pone.0001855
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发表时间：
2008-03-26
期刊：
影响因子：
3.7
通讯作者：
Deloron P
中科院分区：
文献类型：
--
作者：
Tuikue Ndam N;Bischoff E;Proux C;Lavstsen T;Salanti A;Guitard J;Nielsen MA;Coppée JY;Gaye A;Theander T;David PH;Deloron P
妊娠相关疟疾（PAM）引起孕产妇贫血和低出生体重是恶性疟原虫疟疾的多种表现之一。感染的红细胞（iEs）可以获得各种粘附特性，介导疟疾的临床严重程度。在毒力和免疫逃避的分子基础上的最新进展有助于确定var2csa是pam特异性的var基因。本研究提出了18个新采集的胎盘恶性疟原虫的全基因组微阵列转录分析。与全基因组相比，位于亚端粒区域的PAM过表达基因比例和预测输出的PAM过表达基因比例均高于预期。本文描述了对PAM具有特异性的新寄生虫分子的鉴定，这些分子可能参与宿主-病原体相互作用和胎盘趋向性。其中一种蛋白PFI1785w被进一步鉴定为一个双外显子PHIST基因的产物，并且在暴露于恶性疟原虫的女性血清样本中比在男性血清样本中更容易被识别出来。这些发现表明，除了VAR2CSA外，PFI1785w等其他寄生虫蛋白可能也参与了PAM的发病机制。这些数据可能对未来的疫苗开发非常有价值。
Pregnancy-associated malaria (PAM) causing maternal anemia and low birth weight is among the multiple manifestations of Plasmodium falciparum malaria. Infected erythrocytes (iEs) can acquire various adhesive properties that mediate the clinical severity of malaria. Recent advances on the molecular basis of virulence and immune evasion have helped identify var2csa as a PAM-specific var gene. The present study presents a genome-wide microarray transcript analysis of 18 P. falciparum parasite isolates freshly collected from the placenta. The proportion of PAM over-expressed genes located in subtelomeric regions as well as that of PAM over-expressed genes predicted to be exported were higher than expected compared to the whole genome. The identification of novel parasite molecules with specificity to PAM and which are likely involved in host-pathogen interactions and placental tropism is described. One of these proteins, PFI1785w, was further characterized as the product of a two-exon PHIST gene, and was more often recognized by serum samples from P. falciparum-exposed women than from men. These findings suggest that other parasite proteins, such as PFI1785w, may contribute beside VAR2CSA to the pathogenesis of PAM. These data may be very valuable for future vaccine development.
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影响因子：
1.5
作者：
Fried, Michal;Hixson, Kim K.;Duffy, Patrick E.
通讯作者：
Duffy, Patrick E.
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.4269/ajtmh.2007.77.22
发表时间：
2007-07-01
影响因子：
3.3
作者：
Beeson, James G.;Ndungu, Francis;Marsh, Kevin
通讯作者：
Marsh, Kevin
影响因子：
56.9
作者：
Fried, M;Duffy, PE
通讯作者：
Duffy, PE
影响因子：
4.4
作者：
Ricke, CH;Staalsoe, T;Hviid, L
通讯作者：
Hviid, L