Dissecting quantitative trait loci for boron efficiency across multiple environments in Brassica napus.
Dissecting quantitative trait loci for boron efficiency across multiple environments in Brassica napus.
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剖析甘蓝型油菜多种环境中硼效率的数量性状基因座。
DOI：
10.1371/journal.pone.0045215
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Meng J
中科院分区：
文献类型：
--
作者：
Zhao Z;Wu L;Nian F;Ding G;Shi T;Zhang D;Shi L;Xu F;Meng J
高产是作物育种的首要目标，硼（B）是植物必需的微量元素。然而，B缺乏导致产量下降是一个世界性的农业问题。甘蓝型油菜是对缺B最敏感的作物之一，不同品种间对缺B反应存在较大的基因型差异。剖析B耐受B缺乏的遗传基础。以甘蓝型油菜为材料，利用两年的双单倍体群体（TNDH）和三年的BQDH群体，对低B和正常B条件下的种子产量及产量相关性状进行了QTL分析。在低硼和正常B条件下，共检测到80个QTL和42个上位性互作，分别对产量、株高、分支数、角果数、粒数、粒重和B效率系数（BEC）的表型变异贡献率分别为4.15-23.16%和0.53-14.38%。QTL的加性效应比上位性互作的加性-加性效应更重要。4个QTL与环境的互作和7个QTL与环境的互作分别贡献了1.27-4.95%和1.17-3.68%的表型变异。低B条件下籽粒产量的SYLB-A2和BEC的BEC-A2的A2染色体区域与已报道的主效QTL BE 1的区域相当。B。Bra 020592和Bra 020595的同源基因定位在A2区，推测是B效率的候选基因。这些发现揭示了B中B效率的复杂遗传基础。油菜。这些结果为B高效基因的精细定位和克隆以及利用分子标记辅助选择（MAS）选育B高效品种奠定了基础。
High yield is the most important goal in crop breeding, and boron (B) is an essential micronutrient for plants. However, B deficiency, leading to yield decreases, is an agricultural problem worldwide. Brassica napus is one of the most sensitive crops to B deficiency, and considerable genotypic variation exists among different cultivars in response to B deficiency. To dissect the genetic basis of tolerance to B deficiency in B. napus, we carried out QTL analysis for seed yield and yield-related traits under low and normal B conditions using the double haploid population (TNDH) by two-year and the BQDH population by three-year field trials. In total, 80 putative QTLs and 42 epistatic interactions for seed yield, plant height, branch number, pod number, seed number, seed weight and B efficiency coefficient (BEC) were identified under low and normal B conditions, singly explaining 4.15–23.16% and 0.53–14.38% of the phenotypic variation. An additive effect of putative QTLs was a more important controlling factor than the additive-additive effect of epistatic interactions. Four QTL-by-environment interactions and 7 interactions between epistatic interactions and the environment contributed to 1.27–4.95% and 1.17–3.68% of the phenotypic variation, respectively. The chromosome region on A2 of SYLB-A2 for seed yield under low B condition and BEC-A2 for BEC in the two populations was equivalent to the region of a reported major QTL, BE1. The B. napus homologous genes of Bra020592 and Bra020595 mapped to the A2 region and were speculated to be candidate genes for B efficiency. These findings reveal the complex genetic basis of B efficiency in B. napus. They provide a basis for the fine mapping and cloning of the B efficiency genes and for breeding B-efficient cultivars by marker-assisted selection (MAS).
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